The Journal of Experimental Medicine
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Published 2 December 2002. doi:10.1084/jem.20021003
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© Rockefeller University Press, 0022-1007/2002/12/1427/ $5.00
The Journal of Experimental Medicine, Volume 196, Number 11, December 2, 2002 1427-1433

Telomerase Activation and Rejuvenation of Telomere Length in Stimulated T Cells Derived from Serially Transplanted Hematopoietic Stem Cells

Richard C. Allsopp, Samuel Cheshier and Irving L. Weissman

Beckman Center, Pathology Department, Stanford University School of Medicine, Stanford University, Stanford, CA 94305

Address correspondence to R.C. Allsopp, Department of Pathology, Rm. B259, Stanford University School of Medicine, Stanford University, Stanford, CA 94305. Phone: 650-723-7389; Fax: 650-723-4034; E-mail: rallsopp{at}stanford.edu

Telomeres shorten in hematopoietic cells, including hematopoietic stem cells (HSCs), during aging and after transplantation, despite the presence of readily detectable levels of telomerase in these cells. In T cells, antigenic stimulation has been shown to result in a marked increase in the level of telomerase activity. We now show that stimulation of T cells derived from serially transplanted HSC results in a telomerase-dependent elongation of telomere length to a size similar to that observed in T cells isolated directly from young mice. Southern analysis of telomere length in resting and anti-CD3/CD28 stimulated donor-derived splenic T cells revealed an increase in telomere size by ~7 kb for the population as a whole. Stimulation of donor-derived T cells from recipients of HSCs from telomerase-deficient mice did not result in regeneration of telomere length, demonstrating a dependence on telomerase. Furthermore, clonal anti-CD3/CD28 stimulation of donor-derived T cells followed by fluorescent in situ hybridization (FISH) analysis of telomeric signal intensity showed that telomeres had increased in size by ~50% for all clonal expansions. Together, these results imply that one role for telomerase in T cells may be to renew or extend replicative potential via the rejuvenation of telomere length.

Key Words: T cell • hematopoietic stem cell • transplantation • telomere • mouse


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