Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 146K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Flaño, E. Articles by Blackman, M. A. Search for Related Content PubMed PubMed Citation Articles by Flaño, E. Articles by Blackman, M. A. Social Bookmarking                            What's this?

-Herpesvirus Latency Is Preferentially Maintained in Splenic Germinal Center and Memory B Cells

Emilio Flaño, In-Jeong Kim, David L. Woodland and Marcia A. Blackman

Trudeau Institute, Saranac Lake, NY 12983

Address correspondence to Marcia A. Blackman, Trudeau Institute, 100 Algonquin Avenue, Saranac Lake, NY 12983. Phone: 518-891-3080; Fax: 518-891-5126; E-mail: mblackman{at}trudeauinstitute.org

The -herpesviruses are oncogenic B cell lymphotrophic viruses that establish life-long latency in the host. Murine -herpesvirus 68 (MHV-68) infection of mice represents a unique system for analyzing -herpesvirus latency in splenic B cells at different stages of infection. After intranasal infection with MHV-68 we analyzed the establishment of latency 14 days after infection, and the maintenance of latency 3 months after infection in different purified subpopulations of B cells in the spleen. The data show that MHV-68 latency is mainly established in germinal center B cells and that long-term latency is preferentially maintained in two different subsets of isotype-switched B cells, germinal center and memory B cells. Cell cycle analysis indicates that MHV-68 is located in both cycling and resting isotype-switched B cells. Analysis of viral gene expression showed that both lytic and latent viral transcripts were differentially expressed in germinal center and memory B cells during long-term latency. Together, these observations suggested that -herpesviruses exploit the B cell life cycle in the spleen.

Key Words: murine herpesvirus 68 • gammaherpesvirinae • viral latency • B lymphocytes • germinal centers

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Collins, C. M., Boss, J. M., Speck, S. H. (2009). Identification of Infected B-Cell Populations by Using a Recombinant Murine Gammaherpesvirus 68 Expressing a Fluorescent Protein. J. Virol. 83: 6484-6493 [Abstract] [Full Text]  
• Chaganti, S., Heath, E. M., Bergler, W., Kuo, M., Buettner, M., Niedobitek, G., Rickinson, A. B., Bell, A. I. (2009). Epstein-Barr virus colonization of tonsillar and peripheral blood B-cell subsets in primary infection and persistence. Blood 113: 6372-6381 [Abstract] [Full Text]  
• Krug, L. T., Collins, C. M., Gargano, L. M., Speck, S. H. (2009). NF-{kappa}B p50 Plays Distinct Roles in the Establishment and Control of Murine Gammaherpesvirus 68 Latency. J. Virol. 83: 4732-4748 [Abstract] [Full Text]  
• Ottinger, M., Pliquet, D., Christalla, T., Frank, R., Stewart, J. P., Schulz, T. F. (2009). The Interaction of the Gammaherpesvirus 68 orf73 Protein with Cellular BET Proteins Affects the Activation of Cell Cycle Promoters. J. Virol. 83: 4423-4434 [Abstract] [Full Text]  
• Cush, S. S., Flano, E. (2009). Protective Antigen-Independent CD8 T Cell Memory Is Maintained during {gamma}-Herpesvirus Persistence. J. Immunol. 182: 3995-4004 [Abstract] [Full Text]  
• Milho, R., Smith, C. M., Marques, S., Alenquer, M., May, J. S., Gillet, L., Gaspar, M., Efstathiou, S., Simas, J. P., Stevenson, P. G. (2009). In vivo imaging of murid herpesvirus-4 infection. J. Gen. Virol. 90: 21-32 [Abstract] [Full Text]  
• Jarousse, N., Chandran, B., Coscoy, L. (2008). Lack of Heparan Sulfate Expression in B-Cell Lines: Implications for Kaposi's Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus 68 Infections. J. Virol. 82: 12591-12597 [Abstract] [Full Text]  
• Li, H., Ikuta, K., Sixbey, J. W., Tibbetts, S. A. (2008). A Replication-Defective Gammaherpesvirus Efficiently Establishes Long-Term Latency in Macrophages but Not in B Cells In Vivo. J. Virol. 82: 8500-8508 [Abstract] [Full Text]  
• Forrest, J. C., Speck, S. H. (2008). Establishment of B-Cell Lines Latently Infected with Reactivation-Competent Murine Gammaherpesvirus 68 Provides Evidence for Viral Alteration of a DNA Damage-Signaling Cascade. J. Virol. 82: 7688-7699 [Abstract] [Full Text]  
• Gangadharan, B., Hoeve, M. A., Allen, J. E., Ebrahimi, B., Rhind, S. M., Dutia, B. M., Nash, A. A. (2008). Murine gammaherpesvirus-induced fibrosis is associated with the development of alternatively activated macrophages. J. Leukoc. Biol. 84: 50-58 [Abstract] [Full Text]  
• Ptaschinski, C., Rochford, R. (2008). Infection of neonates with murine gammaherpesvirus 68 results in enhanced viral persistence in lungs and absence of infectious mononucleosis syndrome. J. Gen. Virol. 89: 1114-1121 [Abstract] [Full Text]  
• Gargano, L. M., Moser, J. M., Speck, S. H. (2008). Role for MyD88 Signaling in Murine Gammaherpesvirus 68 Latency. J. Virol. 82: 3853-3863 [Abstract] [Full Text]  
• Kayhan, B., Yager, E. J., Lanzer, K., Cookenham, T., Jia, Q., Wu, T.-T., Woodland, D. L., Sun, R., Blackman, M. A. (2007). A Replication-Deficient Murine {gamma}-Herpesvirus Blocked in Late Viral Gene Expression Can Establish Latency and Elicit Protective Cellular Immunity. J. Immunol. 179: 8392-8402 [Abstract] [Full Text]  
• Cush, S. S., Anderson, K. M., Ravneberg, D. H., Weslow-Schmidt, J. L., Flano, E. (2007). Memory Generation and Maintenance of CD8+ T Cell Function during Viral Persistence. J. Immunol. 179: 141-153 [Abstract] [Full Text]  
• Steed, A., Buch, T., Waisman, A., Virgin, H. W. IV (2007). Gamma Interferon Blocks Gammaherpesvirus Reactivation from Latency in a Cell Type-Specific Manner. J. Virol. 81: 6134-6140 [Abstract] [Full Text]  
• Kim, I.-J., Burkum, C. E., Cookenham, T., Schwartzberg, P. L., Woodland, D. L., Blackman, M. A. (2007). Perturbation of B Cell Activation in SLAM-Associated Protein-Deficient Mice Is Associated with Changes in Gammaherpesvirus Latency Reservoirs. J. Immunol. 178: 1692-1701 [Abstract] [Full Text]  
• Gillet, L., Gill, M. B., Colaco, S., Smith, C. M., Stevenson, P. G. (2006). Murine gammaherpesvirus-68 glycoprotein B presents a difficult neutralization target to monoclonal antibodies derived from infected mice. J. Gen. Virol. 87: 3515-3527 [Abstract] [Full Text]  
• Rodrigues, L., Pires de Miranda, M., Caloca, M. J., Bustelo, X. R., Simas, J. P. (2006). Activation of Vav by the Gammaherpesvirus M2 Protein Contributes to the Establishment of Viral Latency in B Lymphocytes.. J. Virol. 80: 6123-6135 [Abstract] [Full Text]  
• Allen, R. D. III, Dickerson, S., Speck, S. H. (2006). Identification of Spliced Gammaherpesvirus 68 LANA and v-Cyclin Transcripts and Analysis of Their Expression In Vivo during Latent Infection. J. Virol. 80: 2055-2062 [Abstract] [Full Text]  
• Madureira, P. A., Matos, P., Soeiro, I., Dixon, L. K., Simas, J. P., Lam, E. W.-F. (2005). Murine {gamma}-Herpesvirus 68 Latency Protein M2 Binds to Vav Signaling Proteins and Inhibits B-cell Receptor-induced Cell Cycle Arrest and Apoptosis in WEHI-231 B Cells. J. Biol. Chem. 280: 37310-37318 [Abstract] [Full Text]  
• Flano, E., Kayhan, B., Woodland, D. L., Blackman, M. A. (2005). Infection of Dendritic Cells by a {gamma}2-Herpesvirus Induces Functional Modulation. J. Immunol. 175: 3225-3234 [Abstract] [Full Text]  
• Moser, J. M., Upton, J. W., Allen, R. D. III, Wilson, C. B., Speck, S. H. (2005). Role of B-Cell Proliferation in the Establishment of Gammaherpesvirus Latency. J. Virol. 79: 9480-9491 [Abstract] [Full Text]  
• Moser, J. M., Upton, J. W., Gray, K. S., Speck, S. H. (2005). Ex Vivo Stimulation of B Cells Latently Infected with Gammaherpesvirus 68 Triggers Reactivation from Latency. J. Virol. 79: 5227-5231 [Abstract] [Full Text]  
• Flano, E., Jia, Q., Moore, J., Woodland, D. L., Sun, R., Blackman, M. A. (2005). Early Establishment of {gamma}-Herpesvirus Latency: Implications for Immune Control. J. Immunol. 174: 4972-4978 [Abstract] [Full Text]  
• Coleman, H. M., Efstathiou, S., Stevenson, P. G. (2005). Transcription of the murine gammaherpesvirus 68 ORF73 from promoters in the viral terminal repeats. J. Gen. Virol. 86: 561-574 [Abstract] [Full Text]  
• Willer, D. O., Speck, S. H. (2005). Establishment and Maintenance of Long-Term Murine Gammaherpesvirus 68 Latency in B Cells in the Absence of CD40. J. Virol. 79: 2891-2899 [Abstract] [Full Text]  
• Herskowitz, J., Jacoby, M. A., Speck, S. H. (2005). The Murine Gammaherpesvirus 68 M2 Gene Is Required for Efficient Reactivation from Latently Infected B Cells. J. Virol. 79: 2261-2273 [Abstract] [Full Text]  
• de Lima, B. D., May, J. S., Marques, S., Simas, J. P., Stevenson, P. G. (2005). Murine gammaherpesvirus 68 bcl-2 homologue contributes to latency establishment in vivo. J. Gen. Virol. 86: 31-40 [Abstract] [Full Text]  
• Simas, J. P., Marques, S., Bridgeman, A., Efstathiou, S., Adler, H. (2004). The M2 gene product of murine gammaherpesvirus 68 is required for efficient colonization of splenic follicles but is not necessary for expansion of latently infected germinal centre B cells. J. Gen. Virol. 85: 2789-2797 [Abstract] [Full Text]  
• McClellan, J. S., Tibbetts, S. A., Gangappa, S., Brett, K. A., Virgin, H. W. IV (2004). Critical Role of CD4 T Cells in an Antibody-Independent Mechanism of Vaccination against Gammaherpesvirus Latency. J. Virol. 78: 6836-6845 [Abstract] [Full Text]  
• de Lima, B. D., May, J. S., Stevenson, P. G. (2004). Murine Gammaherpesvirus 68 Lacking gp150 Shows Defective Virion Release but Establishes Normal Latency In Vivo. J. Virol. 78: 5103-5112 [Abstract] [Full Text]  
• Hochberg, D., Souza, T., Catalina, M., Sullivan, J. L., Luzuriaga, K., Thorley-Lawson, D. A. (2004). Acute Infection with Epstein-Barr Virus Targets and Overwhelms the Peripheral Memory B-Cell Compartment with Resting, Latently Infected Cells. J. Virol. 78: 5194-5204 [Abstract] [Full Text]  
• Flano, E., Hardy, C. L., Kim, I.-J., Frankling, C., Coppola, M. A., Nguyen, P., Woodland, D. L., Blackman, M. A. (2004). T Cell Reactivity during Infectious Mononucleosis and Persistent Gammaherpesvirus Infection in Mice. J. Immunol. 172: 3078-3085 [Abstract] [Full Text]  
• May, J. S., Coleman, H. M., Smillie, B., Efstathiou, S., Stevenson, P. G. (2004). Forced lytic replication impairs host colonization by a latency-deficient mutant of murine gammaherpesvirus-68. J. Gen. Virol. 85: 137-146 [Abstract] [Full Text]  
• Fowler, P., Marques, S., Simas, J. P., Efstathiou, S. (2003). ORF73 of murine herpesvirus-68 is critical for the establishment and maintenance of latency. J. Gen. Virol. 84: 3405-3416 [Abstract] [Full Text]  
• Macrae, A. I., Usherwood, E. J., Husain, S. M., Flano, E., Kim, I.-J., Woodland, D. L., Nash, A. A., Blackman, M. A., Sample, J. T., Stewart, J. P. (2003). Murid Herpesvirus 4 Strain 68 M2 Protein Is a B-Cell-Associated Antigen Important for Latency but Not Lymphocytosis. J. Virol. 77: 9700-9709 [Abstract] [Full Text]  
• Willer, D. O., Speck, S. H. (2003). Long-Term Latent Murine Gammaherpesvirus 68 Infection Is Preferentially Found within the Surface Immunoglobulin D-Negative Subset of Splenic B Cells In Vivo. J. Virol. 77: 8310-8321 [Abstract] [Full Text]  
• Kim, I.-J., Flano, E., Woodland, D. L., Lund, F. E., Randall, T. D., Blackman, M. A. (2003). Maintenance of Long Term {gamma}-Herpesvirus B Cell Latency Is Dependent on CD40-Mediated Development of Memory B Cells. J. Immunol. 171: 886-892 [Abstract] [Full Text]  
• Marques, S., Efstathiou, S., Smith, K. G., Haury, M., Simas, J. P. (2003). Selective Gene Expression of Latent Murine Gammaherpesvirus 68 in B Lymphocytes. J. Virol. 77: 7308-7318 [Abstract] [Full Text]  
• van Dyk, L. F., Virgin, H. W. IV, Speck, S. H. (2003). Maintenance of Gammaherpesvirus Latency Requires Viral Cyclin in the Absence of B Lymphocytes. J. Virol. 77: 5118-5126 [Abstract] [Full Text]  
• Flano, E., Kim, I.-J., Moore, J., Woodland, D. L., Blackman, M. A. (2003). Differential {gamma}-Herpesvirus Distribution in Distinct Anatomical Locations and Cell Subsets During Persistent Infection in Mice. J. Immunol. 170: 3828-3834 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS