The Journal of Experimental Medicine
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Published online 24 June 2002 doi:10.1084/jem.20020201
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© Rockefeller University Press, 0022-1007/2002/7/65/ $5.00
The Journal of Experimental Medicine, Volume 196, Number 1, July 1, 2002 65-75

Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches

Takaharu Okada1, Vu N. Ngo1, Eric H. Ekland1, Reinhold Förster2, Martin Lipp3, Dan R. Littman4 and Jason G. Cyster1

1 Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143
2 Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany
3 Molecular Tumorgenetics and Immunogenetics, Max-Delbrück-Center for Molecular Medicine, 13092 Berlin, Germany
4 Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY 10016

Address correspondence to Jason G. Cyster, Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143. Phone: 415-502-6427; Fax: 415-502-8424; E-mail: cyster{at}itsa.ucsf.edu

B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4-/- B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.

Key Words: chemokine • high endothelial venule • adhesion • lymphoid organ • B cell


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