Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 573K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, X. Articles by Jensen, P. E. Search for Related Content PubMed PubMed Citation Articles by Chen, X. Articles by Jensen, P. E. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Social Bookmarking                            What's this?

Regulated Expression of Human Histocompatibility Leukocyte Antigen (HLA)-DO During Antigen-dependent and Antigen-independent Phases of B Cell Development

Xinjian Chen¹, Oskar Laur¹, Taku Kambayashi¹, Shiyong Li¹, Robert A. Bray¹, Dominique A. Weber¹, Lars Karlsson² and Peter E. Jensen¹

¹ Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322
² The R.W. Johnson Pharmaceutical Research Institute, La Jolla, CA 92037

Address correspondence to Peter E. Jensen, Department of Pathology, Emory University School of Medicine, Room 7313 WMB, 1639 Pierce Drive, Atlanta, GA 30322. Phone: 404-727-3658; Fax: 404-727-5764; E-mail: pjensen{at}emory.edu

Human histocompatibility leukocyte antigen (HLA)-DO, a lysosomal resident major histocompatibility complex class II molecule expressed in B cells, has previously been shown to be a negative regulator of HLA-DM peptide loading function. We analyze the expression of DO in human peripheral blood, lymph node, tonsil, and bone marrow to determine if DO expression is modulated in the physiological setting. B cells, but not monocytes or monocyte-derived dendritic cells, are observed to express this protein. Preclearing experiments demonstrate that 50% of HLA-DM is bound to DO in peripheral blood B cells. HLA-DM and HLA-DR expression is demonstrated early in B cell development, beginning at the pro-B stage in adult human bone marrow. In contrast, DO expression is initiated only after B cell development is complete. In all situations, there is a striking correlation between intracellular DO expression and cell surface class II–associated invariant chain peptide expression, which suggests that DO substantially inhibits DM function in primary human B cells. We report that the expression of DO is markedly downmodulated in human germinal center B cells. Modulation of DO expression may provide a mechanism to regulate peptide loading activity and antigen presentation by B cells during the development of humoral immune responses.

Key Words: antigen presentation • B cell development • germinal center • HLA-DM • HLA-DO

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Fallas, J. L., Yi, W., Draghi, N. A., O'Rourke, H. M., Denzin, L. K. (2007). Expression Patterns of H2-O in Mouse B Cells and Dendritic Cells Correlate with Cell Function. J. Immunol. 178: 1488-1497 [Abstract] [Full Text]  
• Hornell, T. M. C., Burster, T., Jahnsen, F. L., Pashine, A., Ochoa, M. T., Harding, J. J., Macaubas, C., Lee, A. W., Modlin, R. L., Mellins, E. D. (2006). Human Dendritic Cell Expression of HLA-DO Is Subset Specific and Regulated by Maturation. J. Immunol. 176: 3536-3547 [Abstract] [Full Text]  
• Chen, X., Reed-Loisel, L. M., Karlsson, L., Jensen, P. E. (2006). H2-O Expression in Primary Dendritic Cells. J. Immunol. 176: 3548-3556 [Abstract] [Full Text]  
• Bellemare-Pelletier, A., Tremblay, J., Beaulieu, S., Boulassel, M.-R., Routy, J.-P., Massie, B., Lapointe, R., Thibodeau, J. (2005). HLA-DO transduced in human monocyte-derived dendritic cells modulates MHC class II antigen processing. J. Leukoc. Biol. 78: 95-105 [Abstract] [Full Text]  
• Chamuleau, M. E. D., Souwer, Y., van Ham, S. M., Zevenbergen, A., Westers, T. M., Berkhof, J., Meijer, C. J. L. M., van de Loosdrecht, A. A., Ossenkoppele, G. J. (2004). Class II-Associated Invariant Chain Peptide Expression on Myeloid Leukemic Blasts Predicts Poor Clinical Outcome. Cancer Res. 64: 5546-5550 [Abstract] [Full Text]  
• Fallas, J. L., Tobin, H. M., Lou, O., Guo, D., Sant'Angelo, D. B., Denzin, L. K. (2004). Ectopic Expression of HLA-DO in Mouse Dendritic Cells Diminishes MHC Class II Antigen Presentation. J. Immunol. 173: 1549-1560 [Abstract] [Full Text]  
• von Bergwelt-Baildon, M., Schultze, J. L., Maecker, B., Menezes, I., Nadler, L. M., Lapointe, R., Thibodeau, J., Hwu, P. (2004). Correspondence re R. Lapointe et al., CD40-stimulated B Lymphocytes Pulsed with Tumor Antigens Are Effective Antigen-presenting Cells That Can Generate Specific T Cells. Cancer Res 2003;63:2836-43.. Cancer Res. 64: 4055-4057 [Full Text]  
• Alfonso, C., Williams, G. S., Han, J.-O., Westberg, J. A., Winqvist, O., Karlsson, L. (2003). Analysis of H2-O Influence on Antigen Presentation by B Cells. J. Immunol. 171: 2331-2337 [Abstract] [Full Text]  
• Hornell, T. M. C., Beresford, G. W., Bushey, A., Boss, J. M., Mellins, E. D. (2003). Regulation of the Class II MHC Pathway in Primary Human Monocytes by Granulocyte-Macrophage Colony-Stimulating Factor. J. Immunol. 171: 2374-2383 [Abstract] [Full Text]  
• Jinquan, T., Jacobi, H. H., Jing, C., Millner, A., Sten, E., Hviid, L., Anting, L., Ryder, L. P., Glue, C., Skov, P. S., Jarman, E., Lamberth, K., Malling, H.-J., Poulsen, L. K. (2003). CCR3 Expression Induced by IL-2 and IL-4 Functioning as a Death Receptor for B Cells. J. Immunol. 171: 1722-1731 [Abstract] [Full Text]  
• Chalouni, C., Banchereau, J., Vogt, A. B., Pascual, V., Davoust, J. (2003). Human germinal center B cells differ from naive and memory B cells by their aggregated MHC class II-rich compartments lacking HLA-DO. Int Immunol 15: 457-466 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS