The Journal of Experimental Medicine
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Published 1 April 2002. doi:10.1084/jem.20011475
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© Rockefeller University Press, 0022-1007/2002/4/919/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 7, April 1, 2002 919-929

Original Article

Major T Cell Progenitor Activity in Bone Marrow–derived Spleen Colonies

Christophe Lancrin1, Elke Schneider2, Florence Lambolez1, Marie-Laure Arcangeli1, Corinne Garcia-Cordier3, Benedita Rocha1 and Sophie Ezine1

1 INSERM U345, Institut Necker
2 CNRS UMR 8603, Hôpital Necker
3 IFR 94 (Institut Fédératif de Recherche Necker-Enfants-Malades), 75730 Paris Cedex 15, France

Address correspondence to S. Ezine, INSERM U345, Institut Necker, 156 rue de Vaugirard, 75730 Paris, Cedex 15, France. Phone: 00-33-14-061-5365; Fax: 00-33-14-061-5580; E-mail: ezine{at}necker.fr

Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin-) subpopulations were distinguished, namely CD44+ Thy1- cells still capable of natural killer generation and transient low-level B cell generation, and T cell–restricted CD44- Thy1+ cells. At a molecular level, frequency of CD3{varepsilon} and preT{alpha} mRNA was very different in each subset. Furthermore, only the CD44- Thy1+ subset have initiated rearrangements in the T cell receptor ß locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.

Key Words: thymus • CFU-S • transcription factor • lymphoid differentiation • commitment


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