The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 11 March 2002. doi:10.1084/jem.20010898
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 253K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valdez, Y.
Right arrow Articles by Townsend, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valdez, Y.
Right arrow Articles by Townsend, S. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© Rockefeller University Press, 0022-1007/2002/3/683/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 6, March 18, 2002 683-694

Original Article

Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8{alpha}+ Dendritic Cells In Vivo

Yanet Valdez, Weiling Mah, Monte M. Winslow, Lixin Xu, Peter Ling and Sarah E. Townsend

The Biomedical Research Centre and Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada

Address correspondence to Sarah E. Townsend, The Biomedical Research Centre and Dept. of Medical Genetics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. Phone: 604-822-7810; Fax: 604-822-7815; E-mail: sarah{at}brc.ubc.ca

Antigen-specific B cells express major histocompatibility complex class II and can present antigen directly to T cells. Adoptive transfer experiments using transgenic B and T cells demonstrated that antigen-specific B cells can also efficiently transfer antigen to another cell for presentation to T cells in vivo. To identify the antigen-presenting cell that receives antigens from B cells, a strategy was developed to follow the traffic of B cell–derived proteins in vivo. B cells were labeled with the fluorescent dye CFSE and loaded with antigen, before adoptive transfer into recipient mice. Populations of splenocytes from the recipient mice were later assayed for the presence of fluorescent proteins and for the ability to activate T cells. A small number of CD8{alpha}+CD4-CD11blo dendritic cells (DCs) contain proteins transferred from B cells and these DCs effectively present antigens derived from the B cells to T cells. The results suggest that CD8{alpha}+ DCs sample the cells and membranes in their environment for presentation to T cells circulating through the T cell zone. This function of CD8{alpha}+ DCs may be relevant to the priming of an immune response or the induction of T cell tolerance.

Key Words: T cells • B cells • dendritic cells • antigen-presenting cells • flow cytometry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS