The Journal of Experimental Medicine
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Published 4 March 2002. doi:10.1084/jem.20011500
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© Rockefeller University Press, 0022-1007/2002/3/593/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 5, March 4, 2002 593-602

Original Article

Lipid Raft Microdomains : A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses



Sina Bavari1, Catharine M. Bosio1, Elizabeth Wiegand1, Gordon Ruthel1, Amy B. Will1, Thomas W. Geisbert1, Michael Hevey1, Connie Schmaljohn1, Alan Schmaljohn1 and M. Javad Aman1,2

1 U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702
2 Clinical Research Management Inc., Frederick, MD 21702

Address correspondence to M. Javad Aman or Sina Bavari, Dept. of Cell Biology and Biochemistry, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Frederick, MD 21702-5011. Phone: 301-619-6727/301-619-4246; Fax: 301-619-2348; E-mail: amanm{at}ncifcrf.gov or bavaris{at}ncifcrf.gov

Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

Key Words: Filovirus • Ebola • rafts • budding • VLP


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