The Immune Response to Pneumococcal Proteins during Experimental Human Carriage

doi:10.1084/jem.20011576

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Published 4 February 2002. doi:10.1084/jem.20011576

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© Rockefeller University Press, 0022-1007/2002/2/359/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 3, February 4, 2002 359-365

Brief Definitive Report

The Immune Response to Pneumococcal Proteins during Experimental Human Carriage

Tera L. McCool¹, Thomas R. Cate², Gregory Moy¹ and Jeffrey N. Weiser¹

¹ Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104
² Respiratory Pathogens Research Unit, Baylor College of Medicine, Houston, TX 77030

Address correspondence to Jeffrey N. Weiser, 402A Johnson Pavilion, Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104-6076. Phone: 215-573-3511; Fax: 215-898-9557; E-mail: weiser{at}mail.med.upenn.edu

Colonization of the nasopharynx is the initial step in all infectionscaused by Streptococcus pneumoniae. The antibody response tocarriage was examined in an experimental model of human colonizationin healthy adults. Asymptomatic colonization was detected in6/14 subjects and continued for up to 122 d. Susceptibilityto carriage did not correlate with total serum immunoglobulin(Ig)G to the homotypic capsular polysaccharide. All of the colonizedsubjects, in contrast, developed a serum IgG and secretory IgAresponse to a 22 kD protein, whereas 7 of 8 subjects who didnot become colonized had preexisting antibody to this protein.Analysis of the 22 kD protein identified it as the NH₂-terminalregion of pneumococcal surface protein A (PspA). Our findingsprovide evidence for the role of antibody to this protein fragmentin preventing pneumococcal carriage by humans.

Key Words: Streptococcus pneumoniae • vaccine • carriage • colonization • pneumococcal surface protein A

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