The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 13 May 2002 doi:10.1084/jem.20011930
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 278K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morón, G.
Right arrow Articles by Leclerc, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morón, G.
Right arrow Articles by Leclerc, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© Rockefeller University Press, 0022-1007/2002/5/1233/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 10, May 20, 2002 1233-1245

CD8{alpha}2 CD11b+ Dendritic Cells Present Exogenous Virus-like Particles to CD8+ T Cells and Subsequently Express CD8{alpha} and CD205 Molecules

Gabriel Morón1, Paloma Rueda2, Ignacio Casal2 and Claude Leclerc1

1 Unité de Biologie des Régulations Immunitaires, Institut Pasteur, 75724 Paris, France
2 Immunología y Génetica Aplicada, SA, 28037 Madrid, Spain

Address correspondence to Claude Leclerc, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 86 18; Fax: 33 1 45 68 85 40; E-mail: cleclerc{at}pasteur.fr

Recombinant porcine parvovirus virus-like particles (PPV-VLPs) are particulate exogenous antigens that induce a strong, specific cytotoxic T lymphocyte (CTL) response in the absence of adjuvant. In the present report, we demonstrate in vivo that dendritic cells (DCs) present PPV-VLPs to CD8+ T cells after intracellular processing. PPV-VLPs are captured by DCs with a high efficacy, which results in the delivery of these exogenous antigens to 50% of the whole spleen DC population. In vivo, a few hours after injection, PPV-VLPs are presented exclusively to CD8+ T cells by CD8{alpha}- DCs, whereas 15 hours later they are presented mainly by CD8{alpha}+ DCs. After PPV-VLPs processing, a fraction of CD11b+ DCs undergo phenotypic changes, i.e., the up-regulation of CD8{alpha} and CD205 and the loss of CD4 molecules on their surface. The failure to detect mRNA coding for CD8{alpha} in CD11b+ DCs suggests that CD8{alpha} expression by these cells is not due to de novo synthesis. In recombination-activating gene knockout mice (Rag-/-), CD11b+ DCs did not express CD8{alpha} and PPV-VLPs presentation by CD8{alpha}+ DCs was severely diminished. These results indicate that both CD8{alpha}- and CD8{alpha}+ DCs play an important role in the induction of CTL responses by exogenous antigens, such as VLP.

Key Words: virus-like particles • dendritic cells • cross-priming • CTL • exogenous antigens


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS