The Journal of Experimental Medicine
Rockland Immunochemicals for Research
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Published 31 December 2001. doi:10.1084/jem.20011432
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© Rockefeller University Press, 0022-1007/2002/1/1/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 1, January 7, 2002 1-14

Original Article

Dendritic Cells Pulsed with Intact Streptococcus pneumoniae Elicit both Protein- and Polysaccharide-specific Immunoglobulin Isotype Responses In Vivo through Distinct Mechanisms

Jesus Colino, Yi Shen and Clifford M. Snapper

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814

Address correspondence to Clifford M. Snapper, Dept. of Pathology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD 20814. Phone: 301-295-3490; Fax: 301-295-1640; E-mail: csnapper{at}usuhs.mil

Immature bone marrow–derived myeloid dendritic cells (BMDCs) are induced to undergo phenotypic maturation and secretion of tumor necrosis factor (TNF)-{alpha}, interleukin (IL)-6, IL-12, and IL-10 when pulsed in vitro with intact Streptococcus pneumoniae. After transfer to naive mice, pulsed BMDCs induce immunoglobulin (Ig) isotype responses specific for both protein and polysaccharide pneumococcal antigens, having in common the requirement for viable BMDCs, T cells, and B7-dependent costimulation in the recipient mice. Whereas primary Ig isotype responses to bacterial proteins uniformly require BMDC expression of major histocompatibility complex class II, CD40, and B7, and the secretion of IL-6, but not IL-12, similar requirements for antipolysaccharide Ig responses were only observed for the IgG1 isotype.

Key Words: immunity • APCs • cellular • antibody formation • Gram-positive bacteria


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