|
||
Brief Definitive Report |
Correspondence to: Michael Dee Gunn, Box 3547, Duke University Medical Center, Durham, NC 27710. Tel:919-681-0840 Fax:919-684-8591 E-mail:michael.gunn{at}duke.edu.
Human plasmacytoid dendritic cells (pDCs) are major producers of IFN
, are activated by CpG motifs, and are believed to enter lymph nodes (LNs) via L-selectin dependent extravasation across high endothelial venules. To identify a similar murine DC type, CD11c+ cells in the LNs of L-selectindeficient and control BALB/c mice were compared, revealing a population of CD11c+CD11b- cells that is reduced 85% in the LNs of L-selectindeficient mice. These cells are Gr-1+B220+CD19-, either CD4+ or CD8+, and localize within T cell zones of LNs. Freshly isolated CD11c+Gr-1+ cells express major histocompatibility complex class II at low levels, display a plasmacytoid morphology, and survive poorly in culture. Their survival is increased and they develop a DC-like morphology in interleukin 3 and CpG. Like human pDCs, CD11c+Gr-1+ cells stimulate T cell proliferation after activation with CpG and produce IFN
after stimulation with influenza virus. These cells also display a strain-specific variation in frequency, being fivefold increased in the LNs of BALB/c relative to C57BL/6 mice. These CD11c+CD11b-B220+Gr-1+ cells appear to be the murine equivalent of human pDCs.
Key Words: dendritic cells, lymph nodes, B220 antigens, L-selectin, interferon type 1
This article has been cited by other articles:
| TABLE OF CONTENTS |
|