Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7-/- Mice

doi:10.1084/jem.194.8.1141

Published 15 October 2001. doi:10.1084/jem.194.8.1141

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© The Rockefeller University Press, 0022-1007/2001/10/1141/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 8, October 15, 2001 1141-1150

Original Article

Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7^-/- Mice

Thiago L. Carvalho^a, Tomaz Mota-Santos^a, Ana Cumano^b, Jocelyne Demengeot^a, and Paulo Vieira^a,b
^a Instituto Gulbenkian de Ciência, 2781 Oeiras, Portugal
^b Unité du Developpement des Lymphocytes (Centre National de la Recherche Scientific, Unité de Recherche Associée 1961), Institut Pasteur, 75724 Paris Cedex 15, France

Correspondence to: Paulo Vieira, Unite du Developpement des Lymphocytes, Institut Pasteur, 25, Rue du Dr Roux, 75724 Paris Cedex 15, France. Tel:33-1-45-68-82-55 Fax:33-1-45-68-89-21 E-mail:pvieira{at}pasteur.fr.

Interleukin 7 is a crucial factor for the development of murineT and B lymphocytes. We now report that, in the absence of interleukin7, B lymphocyte production takes place exclusively during fetaland perinatal life, ceasing after 7 wk of age. In peripheralorgans, however, the pool of B lymphocytes is stable throughoutadult life and consists only of cells that belong to the B1and marginal zone (MZ) compartments. This is accompanied bya 50-fold increase in the frequency of immunoglobulin (Ig)M-and IgG-secreting cells, and the concentration of serum immunoglobulinsis increased three- to fivefold. Both the MZ phenotype and theincrease in serum IgM are T cell independent. These findingsreveal a previously undescribed pathway of B lymphopoiesis thatis active in early life and is interleukin 7 independent. Thispathway generates B1 cells and a normal sized MZ B lymphocytecompartment.

Key Words: B lymphocyte, interleukin 7, marginal zone B cells, B1 cells,B cell development

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