The Journal of Experimental Medicine
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Published 15 October 2001. doi:10.1084/jem.194.8.1141
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© The Rockefeller University Press, 0022-1007/2001/10/1141/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 8, October 15, 2001 1141-1150

Original Article

Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7–/– Mice

Thiago L. Carvalhoa, Tomaz Mota-Santosa, Ana Cumanob, Jocelyne Demengeota, and Paulo Vieiraa,b

a Instituto Gulbenkian de Ciência, 2781 Oeiras, Portugal
b Unité du Developpement des Lymphocytes (Centre National de la Recherche Scientific, Unité de Recherche Associée 1961), Institut Pasteur, 75724 Paris Cedex 15, France
Unite du Developpement des Lymphocytes, Institut Pasteur, 25, Rue du Dr Roux, 75724 Paris Cedex 15, France.33-1-45-68-89-2133-1-45-68-82-55

pvieira{at}pasteur.fr

Interleukin 7 is a crucial factor for the development of murine T and B lymphocytes. We now report that, in the absence of interleukin 7, B lymphocyte production takes place exclusively during fetal and perinatal life, ceasing after 7 wk of age. In peripheral organs, however, the pool of B lymphocytes is stable throughout adult life and consists only of cells that belong to the B1 and marginal zone (MZ) compartments. This is accompanied by a 50-fold increase in the frequency of immunoglobulin (Ig)M- and IgG-secreting cells, and the concentration of serum immunoglobulins is increased three- to fivefold. Both the MZ phenotype and the increase in serum IgM are T cell independent. These findings reveal a previously undescribed pathway of B lymphopoiesis that is active in early life and is interleukin 7 independent. This pathway generates B1 cells and a normal sized MZ B lymphocyte compartment.

Key Words: B lymphocyte • interleukin 7 • marginal zone B cells • B1 cells • B cell development

Abbreviations used in this paper: FO, follicular; FSC, forward scatter; HSA, heat stable antigen; MZ, marginal zone.

T.L. Carvalho and T. Mota-Santos contributed equally to this work.

T. Mota-Santos' present address is Departamento de Bioquimica e Imunologia, Instituto de Ciências, Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 30161-970, Brazil.

© 2001 The Rockefeller University Press


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