The Journal of Experimental Medicine
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Published online 3 September 2001. doi:10.1084/jem.194.5.557
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© The Rockefeller University Press, 0022-1007/2001/9/557/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 5, September 3, 2001 557-570

Original Article

Joining Chain–Expressing and–Nonexpressing B Cell Populations in the Mouse

Lena Erlandssona, Peter Akerblada, Carina Vingsbo-Lundberga, Eva Kallberga, Nils Lyckeb, and Tomas Leandersona

a Immunology Section, Department of Cell and Molecular Biology, Lund University, S-221 84 Lund, Sweden
b Department of Medical Microbiology and Immunology, University of Goteborg, S-413 46 Goteborg, Sweden
Immunology Section, BMC I:13, Lund University, S-221 84 Lund, Sweden.46-46-222-421846-46-222-4160

Tomas.Leanderson{at}immuno.lu.se

The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain–expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription–polymerase chain reaction showed that J chain–nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes.

Key Words: B cells • plasma cells • mice • ES cells • J chain

Abbreviations used in this paper: CT, Cholera toxin; DTA, Diphtheria toxin A chain; ELISPOT, enzyme-linked immunospot; ES, embryonic stem; HRP, horseradish peroxidase; J chain, Joining chain; LP, lamina propria; RT, reverse transcription; wt, wild-type.

© 2001 The Rockefeller University Press


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