Interferon {gamma} Stabilizes the T Helper Cell Type 1 Phenotype

doi:10.1084/jem.194.2.165

Published online 16 July 2001. doi:10.1084/jem.194.2.165

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© The Rockefeller University Press, 0022-1007/2001/7/165/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 2, July 16, 2001 165-172

Original Article

Interferon ${gamma}$ Stabilizes the T Helper Cell Type 1 Phenotype

Yongkang Zhang^a, Ron Apilado^a, John Coleman^a, Shlomo Ben-Sasson^b^,c, Sharon Tsang^b, Jane Hu-Li^b, William E. Paul^b, and Hua Huang^a

^a The Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153
^b The Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
^c The Lautenberg Center for General and Tumor Immunology, The Hebrew University School of Medicine, Jerusalem 91120, Israel
Dept. of Cell Biology, Neurobiology, and Anatomy, Loyola University Chicago, Stritch School of Medicine, Bldg. 102, Rm. 5657, 2160 South First Ave., Maywood, IL 60153.708-216-3913708-216-3349

hhuang{at}lumc.edu

T helper cell (Th)1-primed CD4 T cells from wild-type donorsmake little interleukin (IL)-4 when restimulated under Th2 conditions.However, such restimulation of Th1-primed cells from interferon(IFN)- ${gamma}$ ^2/– or IFN- ${gamma}$ receptor (IFN- ${gamma}$ R)^–/– miceresulted in substantial production of IL-4 and other Th2 cytokines.Adding IFN- ${gamma}$ to the priming culture markedly diminished the capacityof Th1-primed IFN- ${gamma}$ ^2/– cells to express IL-4. Even IFN- ${gamma}$ –producingcells from IFN- ${gamma}$ R^–/– mice could acquire IL-4–producingcapacity. Thus, IFN- ${gamma}$ is not required for the development ofIFN- ${gamma}$ –producing capacity, but it plays a critical rolein suppressing the IL-4–producing potential of Th1 cells.

Key Words: IL-4 • IL-12 • differentiation • commitment • cell cloning

Abbreviations used in this paper: Jak, Janus kinase; RPA, RNaseprotection assay; SOCS, suppressor of cytokine signaling; STAT,signal transducer and activator of transcription; WT, wild-type.

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