Original Article

Interferon Stabilizes the T Helper Cell Type 1 Phenotype

Correspondence to: Hua Huang, Dept. of Cell Biology, Neurobiology, and Anatomy, Loyola University Chicago, Stritch School of Medicine, Bldg. 102, Rm. 5657, 2160 South First Ave., Maywood, IL 60153. Tel:708-216-3349 Fax:708-216-3913 E-mail:hhuang{at}lumc.edu.

T helper cell (Th)1-primed CD4 T cells from wild-type donors make little interleukin (IL)-4 when restimulated under Th2 conditions. However, such restimulation of Th1-primed cells from interferon (IFN)-^2/- or IFN- receptor (IFN-R)^-/- mice resulted in substantial production of IL-4 and other Th2 cytokines. Adding IFN- to the priming culture markedly diminished the capacity of Th1-primed IFN-^2/- cells to express IL-4. Even IFN-–producing cells from IFN-R^-/- mice could acquire IL-4–producing capacity. Thus, IFN- is not required for the development of IFN-–producing capacity, but it plays a critical role in suppressing the IL-4–producing potential of Th1 cells.

Key Words: IL-4, IL-12, differentiation, commitment, cell cloning

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