The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: J. Exp. Med. 194 (12) 1885
Published 19 November 2001. doi:10.1084/jem.194.10.1519
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 284K)
Right arrow PPT slides of all figures
Right arrow Correction (v194,p1885)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sjöström, A.
Right arrow Articles by Höglund, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sjöström, A.
Right arrow Articles by Höglund, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© Rockefeller University Press, 0022-1007/2001/11/1519/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 10, November 19, 2001 1519-1530

Original Article

Acquisition of External Major Histocompatibility Complex Class I Molecules by Natural Killer Cells Expressing Inhibitory Ly49 Receptors

Anna Sjöström1, Mikael Eriksson2, Cristina Cerboni1, Maria H. Johansson1, Charles L. Sentman2, Klas Kärre1 and Petter Höglund1

1 Microbiology and Tumor Biology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden
2 Umeå Center for Molecular Pathogenesis, Umeå University, S-901 87 Umeå, Sweden

Address correspondence to Dr. Petter Höglund, Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, S–171 77 Stockholm, Sweden. Phone: 46-8-728-62-01; Fax: 46-8-30-42-76; E-mail: petter.hoglund{at}mtc.ki.se

Murine natural killer (NK) cells express inhibitory Ly49 receptors specific for major histocompatibility complex (MHC) class I molecules. We report that during interactions with cells in the environment, NK cells acquired MHC class I ligands from surrounding cells in a Ly49-specific fashion and displayed them at the cell surface. Ligand acquisition sometimes reached 20% of the MHC class I expression on surrounding cells, involved transfer of the entire MHC class I protein to the NK cell, and was independent of whether or not the NK cell expressed the MHC class I ligand itself. We also present indirect evidence for spontaneous MHC class I acquisition in vivo, as well as describe an in vitro coculture system with transfected cells in which the same phenomenon occurred. Functional studies in the latter model showed that uptake of H-2Dd by Ly49A+ NK cells was accompanied by a partial inactivation of cytotoxic activity in the NK cell, as tested against H-2Dd-negative target cells. In addition, ligand acquisition did not abrogate the ability of Ly49A+ NK cells to receive inhibitory signals from external H-2Dd molecules. This study is the first to describe ligand acquisition by NK cells, which parallels recently described phenomena in T and B cells.

Key Words: immunology • cellular immunity • natural immunity • cell communication • biological adaptation


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS