Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 213K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhou, M. Articles by Murphy, K. M. Search for Related Content PubMed PubMed Citation Articles by Zhou, M. Articles by Murphy, K. M. Social Bookmarking                            What's this?

Original Article

¹ Department of Pathology and Immunology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110
² Division of Rheumatology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110
³ Division of Hematology/Oncology, The Children's Hospital Medical Center, and Dana Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA 02115

Address correspondence to Kenneth M. Murphy, Dept. of Pathology and Immunology, Howard Hughes Medical Institute, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110. Phone: 314-362-2009; Fax: 314-747-4888; E-mail: Murphy{at}immunology.wustl.edu

The development of naive CD4⁺ T cells into a T helper (Th) 2 subset capable of producing interleukin (IL)-4, IL-5, and IL-13 involves a signal transducer and activator of transcription (Stat)6-dependent induction of GATA-3 expression, followed by Stat6-independent GATA-3 autoactivation. The friend of GATA (FOG)-1 protein regulates GATA transcription factor activity in several stages of hematopoietic development including erythrocyte and megakaryocyte differentiation, but whether FOG-1 regulates GATA-3 in T cells is uncertain. We show that FOG-1 can repress GATA-3–dependent activation of the IL-5 promoter in T cells. Also, FOG-1 overexpression during primary activation of naive T cells inhibited Th2 development in CD4⁺ T cells. FOG-1 fully repressed GATA-3–dependent Th2 development and GATA-3 autoactivation, but not Stat6-dependent induction of GATA-3. FOG-1 overexpression repressed development of Th2 cells from naive T cells, but did not reverse the phenotype of fully committed Th2 cells. Thus, FOG-1 may be one factor capable of regulating the Th2 development.

Key Words: GATA-3 • FOG-1 • thymocyte • T lymphocyte • cytokine

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Snow, J. W., Orkin, S. H. (2009). Translational Isoforms of FOG1 Regulate GATA1-interacting Complexes. J. Biol. Chem. 284: 29310-29319 [Abstract] [Full Text]  
• Ray, S., Dutta, D., Rumi, M. A. K., Kent, L. N., Soares, M. J., Paul, S. (2009). Context-dependent Function of Regulatory Elements and a Switch in Chromatin Occupancy between GATA3 and GATA2 Regulate Gata2 Transcription during Trophoblast Differentiation. J. Biol. Chem. 284: 4978-4988 [Abstract] [Full Text]  
• Scheinman, E. J., Avni, O. (2009). Transcriptional Regulation of Gata3 in T Helper Cells by the Integrated Activities of Transcription Factors Downstream of the Interleukin-4 Receptor and T Cell Receptor. J. Biol. Chem. 284: 3037-3048 [Abstract] [Full Text]  
• Zhao, X., Zheng, B., Huang, Y., Yang, D., Katzman, S., Chang, C., Fowell, D., Zeng, W.-p. (2007). Interaction between GATA-3 and the Transcriptional Coregulator Pias1 Is Important for the Regulation of Th2 Immune Responses. J. Immunol. 179: 8297-8304 [Abstract] [Full Text]  
• Sundrud, M. S., VanCompernolle, S. E., Eger, K. A., Bruno, T. C., Subramaniam, A., Mummidi, S., Ahuja, S. K., Unutmaz, D. (2005). Transcription factor GATA-1 potently represses the expression of the HIV-1 coreceptor CCR5 in human T cells and dendritic cells. Blood 106: 3440-3448 [Abstract] [Full Text]  
• Tykocinski, L.-O., Hajkova, P., Chang, H.-D., Stamm, T., SOzeri, O., LOhning, M., Hu-Li, J., Niesner, U., Kreher, S., Friedrich, B., Pannetier, C., Grutz, G., Walter, J., Paul, W. E., Radbruch, A. (2005). A Critical Control Element for Interleukin-4 Memory Expression in T Helper Lymphocytes. J. Biol. Chem. 280: 28177-28185 [Abstract] [Full Text]  
• Jenks, S. A., Eisfelder, B. J., Miller, J. (2005). LFA-1 co-stimulation inhibits Th2 differentiation by down-modulating IL-4 responsiveness. Int Immunol 17: 315-323 [Abstract] [Full Text]  
• Marusina, A. I., Kim, D.-K., Lieto, L. D., Borrego, F., Coligan, J. E. (2005). GATA-3 Is an Important Transcription Factor for Regulating Human NKG2A Gene Expression. J. Immunol. 174: 2152-2159 [Abstract] [Full Text]  
• Lin, A. C., Roche, A. E., Wilk, J., Svensson, E. C. (2004). The N Termini of Friend of GATA (FOG) Proteins Define a Novel Transcriptional Repression Motif and a Superfamily of Transcriptional Repressors. J. Biol. Chem. 279: 55017-55023 [Abstract] [Full Text]  
• Jankovic, D., Kullberg, M. C., Caspar, P., Sher, A. (2004). Parasite-Induced Th2 Polarization Is Associated with Down-Regulated Dendritic Cell Responsiveness to Th1 Stimuli and a Transient Delay in T Lymphocyte Cycling. J. Immunol. 173: 2419-2427 [Abstract] [Full Text]  
• Bancroft, A. J., Humphreys, N. E., Worthington, J. J., Yoshida, H., Grencis, R. K. (2004). WSX-1: A Key Role in Induction of Chronic Intestinal Nematode Infection. J. Immunol. 172: 7635-7641 [Abstract] [Full Text]  
• Lu, B., Zagouras, P., Fischer, J. E., Lu, J., Li, B., Flavell, R. A. (2004). Kinetic analysis of genomewide gene expression reveals molecule circuitries that control T cell activation and Th1/2 differentiation. Proc. Natl. Acad. Sci. USA 101: 3023-3028 [Abstract] [Full Text]  
• Katz, S. G., Williams, A., Yang, J., Fujiwara, Y., Tsang, A. P., Epstein, J. A., Orkin, S. H. (2003). Endothelial lineage-mediated loss of the GATA cofactor Friend of GATA 1 impairs cardiac development. Proc. Natl. Acad. Sci. USA 100: 14030-14035 [Abstract] [Full Text]  
• Cho, J. Y., Grigura, V., Murphy, T. L., Murphy, K. (2003). Identification of cooperative monomeric Brachyury sites conferring T-bet responsiveness to the proximal IFN-{gamma} promoter. Int Immunol 15: 1149-1160 [Abstract] [Full Text]  
• Sundrud, M. S., Grill, S. M., Ni, D., Nagata, K., Alkan, S. S., Subramaniam, A., Unutmaz, D. (2003). Genetic Reprogramming of Primary Human T Cells Reveals Functional Plasticity in Th Cell Differentiation. J. Immunol. 171: 3542-3549 [Abstract] [Full Text]  
• Kaufman, C. K., Zhou, P., Pasolli, H. A., Rendl, M., Bolotin, D., Lim, K.-C., Dai, X., Alegre, M.-L., Fuchs, E. (2003). GATA-3: an unexpected regulator of cell lineage determination in skin. Genes Dev. 17: 2108-2122 [Abstract] [Full Text]  
• Komine, O., Hayashi, K., Natsume, W., Watanabe, T., Seki, Y., Seki, N., Yagi, R., Sukzuki, W., Tamauchi, H., Hozumi, K., Habu, S., Kubo, M., Satake, M. (2003). The Runx1 Transcription Factor Inhibits the Differentiation of Naive CD4+ T Cells into the Th2 Lineage by Repressing GATA3 Expression. JEM 198: 51-61 [Abstract] [Full Text]  
• Hofer, T., Nathansen, H., Lohning, M., Radbruch, A., Heinrich, R. (2002). GATA-3 transcriptional imprinting in Th2 lymphocytes: A mathematical model. Proc. Natl. Acad. Sci. USA 99: 9364-9368 [Abstract] [Full Text]  
• Kurata, H., Lee, H.-J., McClanahan, T., Coffman, R. L., O'Garra, A., Arai, N. (2002). Friend of GATA Is Expressed in Naive Th Cells and Functions As a Repressor of GATA-3-Mediated Th2 Cell Development. J. Immunol. 168: 4538-4545 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS