The Journal of Experimental Medicine
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Published online 7 May 2001.
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© The Rockefeller University Press, 0022-1007/2001/5/1087/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 9, May 7, 2001 1087-1096

Original Article

Signal Transducer and Activator of Transcription 6 Controls Chemokine Production and T Helper Cell Type 2 Cell Trafficking in Allergic Pulmonary Inflammation

Anuja Mathewa, James A. MacLeana, Elliot DeHaana, Andrew M. Tagera, Francis H.Y. Greenb, and Andrew D. Lustera

a Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114
b Department of Pathology, University of Calgary, Calgary, Alberta, Canada T2N 1N4
Massachusetts General Hospital, Bldg. 149, 13th St., Charlestown, MA 02129.617-726-5651617-726-5710

luster{at}helix.mgh.harvard.edu

Antigen-specific CD4 T helper type 2 (Th2) cells play a pivotal role in the induction of allergic asthma, but the mechanisms regulating their recruitment into the airways are unknown. Signal transducer and activator of transcription factor (Stat)6 is a transcription factor essential for Th2 cell differentiation. Here we show that Stat6 also controls Th2 cell recruitment and effector function in allergic inflammation in vivo. To isolate the role of Stat6 in regulating Th2 cell trafficking and effector function from its role in Th2 cell differentiation, we used a murine model of asthma in which in vitro–differentiated Stat6+/+ antigen-specific Th2 cells were adoptively transferred into naive Stat6–/– and Stat6+/+ mice followed by aerosol antigen challenge. We found that all of the features of asthma, including Th2 cell accumulation, Th2 and eosinophil-active chemokine production, and airway eosinophilia, mucus production, and hyperresponsiveness seen in Stat6+/+ mice, were dramatically absent in Stat6–/– mice that received Stat6+/+ antigen-specific Th2 cells. Our findings establish Stat6 as essential for Th2 cell trafficking and effector function and suggest that interruption of Stat6 signaling in resident cells of the lung is a novel approach to asthma therapy.

Key Words: asthma • cytokines • eosinophil • transcription factor • knockout mouse

Abbreviations used in this paper: AHR, airway hyperresponsiveness; BAL, bronchoalveolar lavage; CCR, CC chemokine receptor; MDC, macrophage-derived chemokine; PAS, periodic acid-Schiff; RPA, RNase protection assay; Stat, signal transducer and activation of transcription; TARC, thymus- and activation-regulated chemokine; TCA, thymus-derived chemotactic agent; VCAM, vascular cell adhesion molecule.

© 2001 The Rockefeller University Press


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