The Journal of Experimental Medicine
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Published online 7 May 2001.
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© The Rockefeller University Press, 0022-1007/2001/5/1035/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 9, May 7, 2001 1035-1044

Original Article

The Adenylate Cyclase Toxin of Bordetella pertussis Binds to Target Cells via the {alpha}Mβ2 Integrin (Cd11b/Cd18)

Pierre Guermonpreza, Nadia Khelefb, Eric Blouind, Philippe Rieud, Paola Ricciardi-Castagnolie, Nicole Guisob, Daniel Ladantc, and Claude Leclerca

a Unit of Biology of Immune Regulations, Institut Pasteur,
b Unit of Bordetella, Institut Pasteur,
c Unit of Cellular Biochemistry, Institut Pasteur,
d Unit 507, Institut National de la Santé et de la Recherche Medicale (INSERM), Department of Nephrology, Necker Hospital, Paris 75015, France
e Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano 20126, Italy
Unit of Biology of Immune Regulation, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris cedex 15, France.33-1-4568-854033-1-4568-8618

cleclerc{at}pasteur.fr

The adenylate cyclase toxin (CyaA) of Bordetella pertussis is a major virulence factor required for the early phases of lung colonization. It can invade eukaryotic cells where, upon activation by endogenous calmodulin, it catalyzes the formation of unregulated cAMP levels. CyaA intoxication leads to evident toxic effects on macrophages and neutrophils. Here, we demonstrate that CyaA uses the {alpha}Mβ2 integrin (CD11b/CD18) as a cell receptor. Indeed, the saturable binding of CyaA to the surface of various hematopoietic cell lines correlated with the presence of the {alpha}Mβ2 integrin on these cells. Moreover, binding of CyaA to various murine cell lines and human neutrophils was specifically blocked by anti-CD11b monoclonal antibodies. The increase of intracellular cAMP level and cell death triggered by CyaA intoxication was also specifically blocked by anti-CD11b monoclonal antibodies. In addition, CyaA bound efficiently and triggered intracellular cAMP increase and cell death in Chinese hamster ovary cells transfected with {alpha}Mβ2 (CD11b/CD18) but not in cells transfected with the vector alone or with the {alpha}Xβ2 (CD11c/CD18) integrin. Thus, the cellular distribution of CD11b, mostly on neutrophils, macrophages, and dendritic and natural killer cells, supports a role for CyaA in disrupting the early, innate antibacterial immune response.

Key Words: Bordetella • adenylate cyclase • integrin • CD11b • toxin

Abbreviations used in this paper: CHO; Chinese hamster ovary; FHA, filamentous hemagglutinin; FSDC, fetal mouse skin dendritic cell; LDH, lactate dehydrogenase; MFI, mean fluorescence intensity.

© 2001 The Rockefeller University Press


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