The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 19 February 2001.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 301K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mundt, C.
Right arrow Articles by Mårtensson, I.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mundt, C.
Right arrow Articles by Mårtensson, I.-L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/2001/2/435/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 4, February 19, 2001 435-446

Original Article

Loss of Precursor B Cell Expansion but Not Allelic Exclusion in VpreB1/VpreB2 Double-Deficient Mice

Cornelia Mundta, Steve Licencea, Takeyuki Shimizub, Fritz Melchersb, and Inga-Lill Mårtenssona

a Developmental Immunology, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
b Basel Institute for Immunology, 4005 Basel, Switzerland
Developmental Immunology, The Babraham Institute, Cambridge CB2 4AT, UK.44-1223-49602244-1223-496469

lill.martensson{at}bbsrc.ac.uk

The pre-B cell receptor consists of immunoglobulin (Ig) µ heavy chains and surrogate light chain, i.e., the VpreB and {lambda}5 proteins. To analyze the role of the two VpreB proteins, mice lacking the VpreB1 and VpreB2 genes were generated. VpreB1–/–VpreB2–/– mice were impaired in their B cell development at the transition from pre-BI to large pre-BII cells. Pre-BII cells did not expand by proliferation, consequently 40-fold less small pre-BII and immature B cells were found in bone marrow, and the generation of immature and mature conventional B cells in spleen appeared reduced. In addition, only low numbers of B-1a cells were detected in the peritoneum. Surprisingly, Ig heavy chain allelic exclusion was still active, apparently ruling out a signaling role of a VpreB1/VpreB2–containing receptor in this process.

Key Words: B cell development • surrogate light chain • pre-B cell receptor • B cell deficiency • B1-a B cells

Abbreviations used in this paper: BCR, B cell receptor; ES, embryonic stem; RAG, recombination activating gene; RT, reverse transcription; SL, surrogate light.

© 2001 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS