The Journal of Experimental Medicine
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Published online 5 February 2001.
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© The Rockefeller University Press, 0022-1007/2001/2/317/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 3, February 5, 2001 317-328

Original Article

C-Jun Nh2-Terminal Kinase (Jnk)1 and Jnk2 Have Similar and Stage-Dependent Roles in Regulating T Cell Apoptosis and Proliferation

Kanaga Sabapathya, Tuula Kallunkib, Jean-Pierre Davida, Isabella Graefc, Michael Karinb, and Erwin F. Wagnera

a Research Institute of Molecular Pathology, Vienna A 1030, Austria
b Department of Pharmacology, School of Medicine, Cancer Center, University of California at San Diego, La Jolla, California 92093
c Department of Pathology, Stanford Medical School, Stanford, California 94305
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, Vienna A 1030, Austria.43-1-798-937043-1-797-30-888

wagner{at}nt.imp.univie.ac.at

Apoptotic and mitogenic stimuli activate c-Jun NH2-terminal kinases (JNKs) in T cells. Although T cells express both JNK1 and JNK2 isozymes, the absence of JNK2 alone can result in resistance to anti-CD3–induced thymocyte apoptosis and defective mature T cell proliferation. Similar defects in thymocyte apoptosis and mature T cell proliferation, the latter due to reduced interleukin 2 production, are also caused by JNK1 deficiency. Importantly, T cell function was compromised in Jnk1+/–Jnk2+/– double heterozygous mice, indicating that JNK1 and JNK2 play similar roles in regulating T cell function. The reduced JNK dose results in defective c-Jun NH2-terminal phosphorylation in thymocytes but not in peripheral T cells, in which nuclear factors of activated T cells (NK-ATs)–DNA binding activity is affected. Thus, JNK1 and JNK2 control similar functions during T cell maturation through differential targeting of distinct substrates.

Key Words: apoptosis • JNK1 • JNK2 • proliferation • T lymphocyte

K. Sabapathy's present address is National Cancer Centre, Division of Cellular and Molecular Research, 11 Hospital Dr., Singapore 169610, Singapore. T. Kallunki's present address is Apoptosis Laboratory, Danish Cancer Society, Strandboulevarden 49, DK-2100, Copenhagen, Denmark.

Abbreviations used in this paper: dn, dominant negative; DP, double positive; ES, embryonic stem; GST, glutathione S-transferase; JNK, c-Jun NH2-terminal kinase; NF-ATc, cyclosporin A–sensitive NF-AT; NF-AT, nuclear factors of activated T cell.

© 2001 The Rockefeller University Press


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