The Journal of Experimental Medicine
for flow cytometry > invitrogen
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 15 January 2001.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 351K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zermati, Y.
Right arrow Articles by Hermine, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zermati, Y.
Right arrow Articles by Hermine, O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/2001/1/247/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 2, January 15, 2001 247-254

Brief Definitive Report

Caspase Activation Is Required for Terminal Erythroid Differentiation

Yael Zermatia, Carmen Garridod, Sophie Amselleme, Serge Fishelsone, Didier Bouscaryf, Françoise Valensib, Bruno Vareta,c, Eric Solaryd, and Olivier Herminea,c

a Centre National de la Recherche Scientifique Unité Mixte de Recherche 8603, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
b Laboratoire d'Hématologie, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
c Service d'Hématologie Clinique, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
d Institut National de la Santé et de la Recherche Médicale (INSERM) U517, Unité Fédérative de Recherche Medecine et Pharmacie, Dijon 21033, France
e Laboratoire de Recherche d'Hémobiologie, Hôpital Cochin, 75014 Paris, France
f INSERM U363, Hôpital Cochin, 75014 Paris, France
Service d'Hématologie Clinique and CNRS UMR 8603, Hôpital Necker, 149-161 rue de Sèvres, 75743 Paris cedex 15, France.33-1-44-49-52-8033-1-44-49-52-83

hermine{at}necker.fr

The cysteine proteases known as caspases play a central role in most apoptotic pathways. Here, we show that caspase inhibitors arrest the maturation of human erythroid progenitors at early stages of differentiation, before nucleus and chromatin condensation. Effector caspases such as caspase-3 are transiently activated through the mitochondrial pathway during erythroblast differentiation and cleave proteins involved in nucleus integrity (lamin B) and chromatin condensation (acinus) without inducing cell death and cleavage of GATA-1. These observations indicate a new function for caspases as key proteases in the process of erythroid differentiation.

Key Words: apoptosis • erythropoiesis • mitochondria • acinus • lamin B

© 2001 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS