The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 16 October 2000.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 156K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sigal, L. J.
Right arrow Articles by Rock, K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sigal, L. J.
Right arrow Articles by Rock, K. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/2000/10/1143/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 8, October 16, 2000 1143-1150

Original Article

Bone Marrow–Derived Antigen-Presenting Cells Are Required for the Generation of Cytotoxic T Lymphocyte Responses to Viruses and Use Transporter Associated with Antigen Presentation (Tap)-Dependent and -Independent Pathways of Antigen Presentation

Luis J. Sigala and Kenneth L. Rocka

a Department of Pathology, University of Massachusetts Medical Center, Worcester, Massachusetts 01655
Fox Chase Cancer Center, Rm. 343, 7701 Burholme Ave., Philadelphia, PA 19111.413-487-5406215-728-7061

Bone marrow (BM)-derived professional antigen-presenting cells (pAPCs) are required for the generation of cytotoxic T lymphocyte (CTL) responses to vaccinia virus and poliovirus. Furthermore, these BM-derived pAPCs require a functional transporter associated with antigen presentation (TAP). In this report we analyze the requirements for BM-derived pAPCs and TAP in the initiation of CTL responses to lymphocytic choriomeningitis virus (LCMV) and influenza virus (Flu). Our results indicate a requirement for BM-derived pAPCs for the CTL responses to these viruses. However, we found that the generation of CTLs to one LCMV epitope (LCMV nucleoprotein 396–404) was dependent on BM-derived pAPCs but, surprisingly, TAP independent. The study of the CTL response to Flu confirmed the existence of this BM-derived pAPC-dependent/TAP-independent CTL response and indicated that the TAP-independent pathway is ~10–300-fold less efficient than the TAP-dependent pathway.

Key Words: mice • lymphocytic choriomeningitis virus • influenza virus • T lymphocytes, cytotoxic • immunity, cellular

Abbreviations used in this paper: B6, C57BL/6; B6D2, (C57BL/6 x DBA2)F1; BM, bone marrow; D2, DBA2; ER, endoplasmic reticulum; Flu, influenza virus; gp, glycoprotein; HAU, hemagglutination unit(s); LCMV, lymphocytic choriomeningitis virus; np, nucleoprotein; pAPC, professional APC; TAP, transporter associated with antigen presentation; TAP0/0, B6-Tap1tp 1Arp.

© 2000 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS