Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

doi:10.1084/jem.192.5.671

Published online 5 September 2000.

This Article

	Full Text
	Full Text (PDF, 177K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Sims, J. E.
	Articles by Paxton, R. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Sims, J. E.
	Articles by Paxton, R. J.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0022-1007/2000/9/671/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 5, September 5, 2000 671-680

Original Article

Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

John E. Sims^a, Douglas E. Williams^a, Philip J. Morrissey^a, Kirsten Garka^a, Diane Foxworthe^a, Virginia Price^a, Sherree L. Friend^b, Andrew Farr^b, Mary A. Bedell^c, Nancy A. Jenkins^c, Neal G. Copeland^c, Kenneth Grabstein^a, and Raymond J. Paxton^a

^a Department of Molecular Biology, Department of Biological Sciences, and the Department of Protein Chemistry, Immunex Corporation, Seattle, Washington 98101
^b Department of Biological Structure, University of Washington, Seattle, Washington 98195
^c Mammalian Genetics Program, National Cancer Institute–Frederick Cancer Research and Development Center, Frederick, Maryland 21702
Immunex Corporation, 51 University St., Seattle, WA 98101.206-233-9733206-587-0430

sims{at}immunex.com

Using a bioassay consisting of the proliferation of a murineB cell line, a cDNA of a gene whose product supports the growthof that cell line was isolated from a thymic stromal cell line.This factor, termed thymic stromal lymphopoietin (TSLP), isa protein of 140 amino acids. The gene encoding TSLP was mappedto murine chromosome 18. Purified recombinant TSLP supportedthe growth of pre-B cell colonies in vitro, but had no myelopoieticactivity. TSLP had comitogenic activity for fetal thymocytes,but was not as potent as interleukin 7 in lobe submersion cultures.Injection of TSLP into neonatal mice induced the expansion ofB220⁺BP-1⁺ pre-B cells.

Key Words: cytokine • B lymphocyte • growth factor • lymphocyte differentiation • DNA sequence

Abbreviations used in this paper: Apc, adenomatosis polyposiscoli; HPRT, hypoxanthine phosphoribosyltransferase; HSA, heat-stableantigen; RP, reversed-phase; RT, reverse transcription; SCF,stem cell factor; sIg, surface Ig; TSLP, thymic stromal lymphopoietin.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ramalingam, T. R., Pesce, J. T., Mentink-Kane, M. M., Madala, S., Cheever, A. W., Comeau, M. R., Ziegler, S. F., Wynn, T. A. (2009). Regulation of Helminth-Induced Th2 Responses by Thymic Stromal Lymphopoietin. J. Immunol. 182: 6452-6459 [Abstract] [Full Text]
Taylor, B. C., Zaph, C., Troy, A. E., Du, Y., Guild, K. J., Comeau, M. R., Artis, D. (2009). TSLP regulates intestinal immunity and inflammation in mouse models of helminth infection and colitis. JEM 206: 655-667 [Abstract] [Full Text]
Chappaz, S., Flueck, L., Farr, A. G., Rolink, A. G., Finke, D. (2007). Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice. Blood 110: 3862-3870 [Abstract] [Full Text]
Brown, V. I., Hulitt, J., Fish, J., Sheen, C., Bruno, M., Xu, Q., Carroll, M., Fang, J., Teachey, D., Grupp, S. A. (2007). Thymic Stromal-Derived Lymphopoietin Induces Proliferation of Pre-B Leukemia and Antagonizes mTOR Inhibitors, Suggesting a Role for Interleukin-7R{alpha} Signaling. Cancer Res. 67: 9963-9970 [Abstract] [Full Text]
Kato, A., Favoreto, S. Jr., Avila, P. C., Schleimer, R. P. (2007). TLR3- and Th2 Cytokine-Dependent Production of Thymic Stromal Lymphopoietin in Human Airway Epithelial Cells. J. Immunol. 179: 1080-1087 [Abstract] [Full Text]
Omori, M., Ziegler, S. (2007). Induction of IL-4 Expression in CD4+ T Cells by Thymic Stromal Lymphopoietin. J. Immunol. 178: 1396-1404 [Abstract] [Full Text]
Lee, H.-C., Ziegler, S. F. (2007). Inducible expression of the proallergic cytokine thymic stromal lymphopoietin in airway epithelial cells is controlled by NF{kappa}B. Proc. Natl. Acad. Sci. USA 104: 914-919 [Abstract] [Full Text]
Hartgring, S A Y, Bijlsma, J W J, Lafeber, F P J G, van Roon, J A G (2006). Interleukin-7 induced immunopathology in arthritis. Ann Rheum Dis 65: iii69-iii74 [Abstract] [Full Text]
Liu, Y.-J. (2006). Thymic stromal lymphopoietin: master switch for allergic inflammation. JEM 203: 269-273 [Abstract] [Full Text]
Li, M., Messaddeq, N., Teletin, M., Pasquali, J.-L., Metzger, D., Chambon, P. (2005). Retinoid X receptor ablation in adult mouse keratinocytes generates an atopic dermatitis triggered by thymic stromal lymphopoietin. Proc. Natl. Acad. Sci. USA 102: 14795-14800 [Abstract] [Full Text]
Al-Shami, A., Spolski, R., Kelly, J., Keane-Myers, A., Leonard, W. J. (2005). A role for TSLP in the development of inflammation in an asthma model. JEM 202: 829-839 [Abstract] [Full Text]
Polli, M., Dakic, A., Light, A., Wu, L., Tarlinton, D. M., Nutt, S. L. (2005). The development of functional B lymphocytes in conditional PU.1 knock-out mice. Blood 106: 2083-2090 [Abstract] [Full Text]
Yoo, J., Omori, M., Gyarmati, D., Zhou, B., Aye, T., Brewer, A., Comeau, M. R., Campbell, D. J., Ziegler, S. F. (2005). Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin. JEM 202: 541-549 [Abstract] [Full Text]
Munitic, I., Williams, J. A., Yang, Y., Dong, B., Lucas, P. J., El Kassar, N., Gress, R. E., Ashwell, J. D. (2004). Dynamic regulation of IL-7 receptor expression is required for normal thymopoiesis. Blood 104: 4165-4172 [Abstract] [Full Text]
Hanson, J. L., Hawke, N. A., Kashatus, D., Baldwin, A. S. (2004). The Nuclear Factor {kappa}B Subunits RelA/p65 and c-Rel Potentiate but Are Not Required for Ras-Induced Cellular Transformation. Cancer Res. 64: 7248-7255 [Abstract] [Full Text]
Kang, J., DiBenedetto, B., Narayan, K., Zhao, H., Der, S. D., Chambers, C. A. (2004). STAT5 Is Required for Thymopoiesis in a Development Stage-Specific Manner. J. Immunol. 173: 2307-2314 [Abstract] [Full Text]
Vosshenrich, C. A. J., Cumano, A., Muller, W., Di Santo, J. P., Vieira, P. (2004). Pre-B cell receptor expression is necessary for thymic stromal lymphopoietin responsiveness in the bone marrow but not in the liver environment. Proc. Natl. Acad. Sci. USA 101: 11070-11075 [Abstract] [Full Text]
Al-Shami, A., Spolski, R., Kelly, J., Fry, T., Schwartzberg, P. L., Pandey, A., Mackall, C. L., Leonard, W. J. (2004). A Role for Thymic Stromal Lymphopoietin in CD4+ T Cell Development. JEM 200: 159-168 [Abstract] [Full Text]
Carpino, N., Thierfelder, W. E., Chang, M.-s., Saris, C., Turner, S. J., Ziegler, S. F., Ihle, J. N. (2004). Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development. Mol. Cell. Biol. 24: 2584-2592 [Abstract] [Full Text]
Osborn, M. J., Ryan, P. L., Kirchhof, N., Panoskaltsis-Mortari, A., Mortari, F., Tudor, K.-S. R. S. (2004). Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis. Blood 103: 843-851 [Abstract] [Full Text]
Gilliet, M., Soumelis, V., Watanabe, N., Hanabuchi, S., Antonenko, S., de Waal-Malefyt, R., Liu, Y.-J. (2003). Human Dendritic Cells Activated by TSLP and CD40L Induce Proallergic Cytotoxic T Cells. JEM 197: 1059-1063 [Abstract] [Full Text]
Dainiak, N., Waselenko, J. K., Armitage, J. O., MacVittie, T. J., Farese, A. M. (2003). The Hematologist and Radiation Casualties. ASH Education Book 2003: 473-496 [Abstract] [Full Text]
Ozaki, K., Leonard, W. J. (2002). Cytokine and Cytokine Receptor Pleiotropy and Redundancy. J. Biol. Chem. 277: 29355-29358 [Full Text]
Fry, T. J., Mackall, C. L. (2002). Interleukin-7: from bench to clinic. Blood 99: 3892-3904 [Full Text]
Isaksen, D. E., Baumann, H., Zhou, B., Nivollet, S., Farr, A. G., Levin, S. D., Ziegler, S. F. (2002). Uncoupling of Proliferation and Stat5 Activation in Thymic Stromal Lymphopoietin-Mediated Signal Transduction. J. Immunol. 168: 3288-3294 [Abstract] [Full Text]
Taneda, S., Segerer, S., Hudkins, K. L., Cui, Y., Wen, M., Segerer, M., Wener, M. H., Khairallah, C. G., Farr, A. G., Alpers, C. E. (2001). Cryoglobulinemic Glomerulonephritis in Thymic Stromal Lymphopoietin Transgenic Mice. Am. J. Pathol. 159: 2355-2369 [Abstract] [Full Text]
Martinez-M., J. A., Minguet, S., Gonzalo, P., Soro, P. G., de Andres, B., Izcue, A., Marcos, M. A. R., Gaspar, M.-L. (2001). Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice. Blood 98: 1862-1871 [Abstract] [Full Text]
Shah, N., Oseth, L., Tran, H., Hirsch, B., LeBien, T. W. (2001). Clonal Variation in the B-Lineage Acute Lymphoblastic Leukemia Response to Multiple Cytokines and Bone Marrow Stromal Cells. Cancer Res. 61: 5268-5274 [Abstract] [Full Text]
Reche, P. A., Soumelis, V., Gorman, D. M., Clifford, T., Liu, M.-r., Travis, M., Zurawski, S. M., Johnston, J., Liu, Y.-J., Spits, H., de Waal Malefyt, R., Kastelein, R. A., Bazan, J. F. (2001). Human Thymic Stromal Lymphopoietin Preferentially Stimulates Myeloid Cells. J. Immunol. 167: 336-343 [Abstract] [Full Text]
Huang, J., Muegge, K. (2001). Control of chromatin accessibility for V(D)J recombination by interleukin-7. J. Leukoc. Biol. 69: 907-911 [Abstract] [Full Text]
Park, L. S., Martin, U., Garka, K., Gliniak, B., Di Santo, J. P., Muller, W., Largaespada, D. A., Copeland, N. G., Jenkins, N. A., Farr, A. G., Ziegler, S. F., Morrissey, P. J., Paxton, R., Sims, J. E. (2000). Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor. JEM 192: 659-670 [Abstract] [Full Text]
Tan, J. T., Dudl, E., LeRoy, E., Murray, R., Sprent, J., Weinberg, K. I., Surh, C. D. (2001). IL-7 is critical for homeostatic proliferation and survival of naive T cells. Proc. Natl. Acad. Sci. USA 98: 8732-8737 [Abstract] [Full Text]