The Journal of Experimental Medicine
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Published online 5 September 2000.
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© The Rockefeller University Press, 0022-1007/2000/9/671/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 5, September 5, 2000 671-680

Original Article

Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

John E. Simsa, Douglas E. Williamsa, Philip J. Morrisseya, Kirsten Garkaa, Diane Foxworthea, Virginia Pricea, Sherree L. Friendb, Andrew Farrb, Mary A. Bedellc, Nancy A. Jenkinsc, Neal G. Copelandc, Kenneth Grabsteina, and Raymond J. Paxtona

a Department of Molecular Biology, Department of Biological Sciences, and the Department of Protein Chemistry, Immunex Corporation, Seattle, Washington 98101
b Department of Biological Structure, University of Washington, Seattle, Washington 98195
c Mammalian Genetics Program, National Cancer Institute–Frederick Cancer Research and Development Center, Frederick, Maryland 21702
Immunex Corporation, 51 University St., Seattle, WA 98101.206-233-9733206-587-0430

sims{at}immunex.com

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.

Key Words: cytokine • B lymphocyte • growth factor • lymphocyte differentiation • DNA sequence

Abbreviations used in this paper: Apc, adenomatosis polyposis coli; HPRT, hypoxanthine phosphoribosyltransferase; HSA, heat-stable antigen; RP, reversed-phase; RT, reverse transcription; SCF, stem cell factor; sIg, surface Ig; TSLP, thymic stromal lymphopoietin.

© 2000 The Rockefeller University Press


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