The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 5 September 2000.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 250K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, L. S.
Right arrow Articles by Sims, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, L. S.
Right arrow Articles by Sims, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/2000/9/659/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 5, September 5, 2000 659-670

Original Article

Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor



Linda S. Parka, Unja Martina, Kirsten Garkaa, Brian Gliniaka, James P. Di Santob, Werner Mullerc, David A. Largaespadad, Neal G. Copelandd, Nancy A. Jenkinsd, Andrew G. Farre, Steven F. Zieglerf, Philip J. Morrisseya, Raymond Paxtona, and John E. Simsa

a Department of Biochemistry, Department of Biological Sciences, and the Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101
b Departement d'Immunologie, Institut Pasteur, 75724 Paris, France
c Institute for Genetics, Cologne D-50931, Germany
d Mammalian Genetics Laboratory, ABL-Basic Research Program National Cancer Institute–Frederick Cancer Research and Development Center, Frederick, Maryland 21702
e Department of Biological Structure, University of Washington, Seattle, Washington 98195
f Virginia Mason Research Center, Seattle, Washington 98101
Immunex Corporation, 51 University St., Seattle, WA 98101.206-233-9733206-587-0430

sims{at}immunex.com

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)R{alpha} chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7R{alpha}–/– mice, but did activate cells from {gamma}c–/– mice. Thus, the functional TSLPR requires the IL-7R{alpha} chain, but not the {gamma}c chain for signaling.

Key Words: cytokine receptors • interleukin 2 receptor • interleukin 7 receptor • cytokine • DNA sequence

Abbreviations used in this paper: Ka, affinity constant; RAG, recombination activating gene; SCF, stem cell factor; TSLP, thymic stromal lymphopoietin.

© 2000 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS