The Journal of Experimental Medicine
IN Cell Analyzer 2000
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Published online 5 September 2000.
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© The Rockefeller University Press, 0022-1007/2000/9/613/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 5, September 5, 2000 613-624

Original Article

Analysis of Qa-1bPeptide Binding Specificity and the Capacity of Cd94/Nkg2a to Discriminate between Qa-1–Peptide Complexes

Jennifer R. Krafta, Russell E. Vancec, Jan Pohlb, Amy M. Martinb, David H. Rauletc, and Peter E. Jensena

a Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322
b Microchemical Facility, Emory University School of Medicine, Atlanta, Georgia 30322
c Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, California 94720
Department of Pathology and Laboratory Medicine, Rm. 7313 WMRB, Emory University School of Medicine, Atlanta, GA 30322.404-727-5764404-727-3658

pjensen{at}bimcore.emory.edu

The major histocompatibility complex class Ib protein, Qa-1b, serves as a ligand for murine CD94/NKG2A natural killer (NK) cell inhibitory receptors. The Qa-1b peptide-binding site is predominantly occupied by a single nonameric peptide, Qa-1 determinant modifier (Qdm), derived from the leader sequence of H-2D and L molecules. Five anchor residues were identified in this study by measuring the peptide-binding affinities of substituted Qdm peptides in experiments with purified recombinant Qa-1b. A candidate peptide-binding motif was determined by sequence analysis of peptides eluted from Qa-1 that had been folded in the presence of random peptide libraries or pools of Qdm derivatives randomized at specific anchor positions. The results indicate that Qa-1b can bind a diverse repertoire of peptides but that Qdm has an optimal primary structure for binding Qa-1b. Flow cytometry experiments with Qa-1b tetramers and NK target cell lysis assays demonstrated that CD94/NKG2A discriminates between Qa-1b complexes containing peptides with substitutions at nonanchor positions P4, P5, or P8. Our findings suggest that it may be difficult for viruses to generate decoy peptides that mimic Qdm and raise the possibility that competitive replacement of Qdm with other peptides may provide a novel mechanism for activation of NK cells.

Key Words: CD94 • NKG2A • Qa-1 • natural killer cell • major histocompatibility complex

Abbreviations used in this paper: β2m, β2-microglobulin; hsp, heat shock protein; Qdm, Qa-1 determinant modifier; TAP, transporter associated with antigen processing.

J.R. Kraft and R.E. Vance contributed equally to this work.

© 2000 The Rockefeller University Press


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