The Journal of Experimental Medicine
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Published online 21 August 2000.
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© The Rockefeller University Press, 0022-1007/2000/8/475/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 4, August 21, 2000 475-482

Original Article

B Lymphocytes Regulate Dendritic Cell (Dc) Function in Vivo: Increased Interleukin 12 Production by DCs from B Cell–Deficient Mice Results in T Helper Cell Type 1 Deviation



Véronique Moulina, Fabienne Andrisa, Kris Thielemansb, Charlie Maliszewskic, Jacques Urbaina, and Muriel Mosera

a Département de Biologie Moléculaire, Université Libre de Bruxelles, 6041 Gosselies, Belgium
b Laboratorium of Hematologie-Immunologie, Vrije Universiteit Brussel, B-1090 Brussels, Belgium
c Immunex Corporation, Seattle, Washington 98101-2936
Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Rue des Prof. Jeener et Brachet, 12; B-6041 Gosselies, Belgium.32-2-650-98-6032-2-650-98-63

mmoser{at}dbm.ulb.ac.be

Increasing evidence indicates that dendritic cells (DCs) are the antigen-presenting cells of the primary immune response. However, several reports suggest that B lymphocytes could be required for optimal T cell sensitization. We compared the immune responses of wild-type and B cell-deficient (µMT) mice, induced by antigen emulsified in adjuvant or pulsed on splenic dendritic cells. Our data show that lymph node cells from both control and µMT animals were primed, but each released distinct cytokine profiles. Lymph node T cells from control animals secreted interferon (IFN)-{gamma}, interleukin (IL)-2, and IL-4, whereas those from µMT mice produced IFN-{gamma} and IL-2 but no IL-4. To test whether B cells may influence the T helper cell type 1 (Th1)/Th2 balance by affecting the function of DCs, we immunized mice by transferring antigen-pulsed DCs from wild-type or mutant mice. Injection of control DCs induced the secretion of IL-4, IFN-{gamma}, and IL-2, whereas administration of DCs from µMT animals failed to sensitize cells to produce IL-4. Analysis of IL-12 production revealed that DCs from µMT mice produce higher levels of IL-12p70 than do DCs from wild-type animals. These data suggest that B lymphocytes regulate the capacity of DCs to promote IL-4 secretion, possibly by downregulating their secretion of IL-12, thereby favoring the induction of a nonpolarized immune response.

Key Words: T helper cell type 1/type 2 balance • primary response • interleukin 4 • interleukin 10 • dendritic–B cell interaction

Abbreviations used in this paper: DC, dendritic cell; µMT, B cell–deficient.

© 2000 The Rockefeller University Press


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