The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 17 July 2000.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 720K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mastroeni, P.
Right arrow Articles by Dougan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mastroeni, P.
Right arrow Articles by Dougan, G.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/2000/7/237/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 2, July 17, 2000 237-248

Original Article

Antimicrobial Actions of the Nadph Phagocyte Oxidase and Inducible Nitric Oxide Synthase in Experimental Salmonellosis. II. Effects on Microbial Proliferation and Host Survival in Vivo

Pietro Mastroenia,b, Andrés Vazquez-Torresc,d,e, Ferric C. Fangc,d,e, Yisheng Xuc,d,e, Shahid Khana, Carlos E. Hormaechea,f, and Gordon Douganb

a Centre for Veterinary Science, University of Cambridge, Cambridge CB3 0ES, United Kingdom
b Department of Biochemistry, Imperial College, London SW7 2AZ, United Kingdom
c Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262
d Department of Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262
e Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262
f Department of Microbiology and Immunology, The Medical School, University of Newcastle, Newcastle upon Tyne, NE2 4HH, United Kingdom
Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK.44-1223-33761044-1223-339868

pm274{at}cm.ac.uk

The roles of the NADPH phagocyte oxidase (phox) and inducible nitric oxide synthase (iNOS) in host resistance to virulent Salmonella typhimurium were investigated in gp91phox–/–, iNOS–/–, and congenic wild-type mice. Although both gp91phox–/– and iNOS–/– mice demonstrated increased susceptibility to infection with S. typhimurium compared with wild-type mice, the kinetics of bacterial replication were dramatically different in the gp91phox–/– and iNOS–/– mouse strains. Greater bacterial numbers were present in the spleens and livers of gp91phox–/– mice compared with C57BL/6 controls as early as day 1 of infection, and all of the gp91phox–/– mice succumbed to infection within 5 d. In contrast, an increased bacterial burden was detected within reticuloendothelial organs of iNOS–/– mice only beyond the first week of infection. Influx of inflammatory CD11b+ cells, granuloma formation, and serum interferon {gamma} levels were unimpaired in iNOS–/– mice, but the iNOS-deficient granulomas were unable to limit bacterial replication. The NADPH phagocye oxidase and iNOS are both required for host resistance to wild-type Salmonella, but appear to operate principally at different stages of infection.

Key Words: Salmonella • virulence • innate immunity • oxidative • nitrosative

Abbreviations used in this paper: iNOS, inducible nitric oxide synthase; LB, Luria-Bertani; NO, nitric oxide; NOS, nitric oxide synthase; RNS, reactive nitrogen species; ROS, reactive oxygen species.

© 2000 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS