The Journal of Experimental Medicine
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Published online 3 July 2000.
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© The Rockefeller University Press, 0022-1007/2000/7/145/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 1, July 3, 2000 145-150

Brief Definitive Reports

Immature Dendritic Cells Acquire Cd8+Cytotoxic T Lymphocyte Priming Capacity upon Activation by T Helper Cell–Independent or–Dependent Stimuli

Danita H. Schuurhuisa, Sandra Labana, René E.M. Toesa, Paola Ricciardi-Castagnolib, Monique J. Kleijmeerc, Ellen I.H. van der Voorta, Delphine Reaa, Rienk Offringaa, Hans J. Geuzec, Cornelis J.M. Meliefa, and Ferry Ossendorpa

a Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
b Department of Biotechnology and Bioscience, University of Milano-Bicocca, 20126 Milano, Italy
c Department of Cell Biology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
Dept. of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, P.O. Box 9600, RC Leiden, The Netherlands.31-71-521675131-71-5263843

f.a.ossendorp{at}LUMC.nl

The well defined, immature murine dendritic cell (DC) line D1 was used to study the role of DC maturation in CTL induction in vitro and in vivo. Maturation of D1 cells, characterized by markedly increased expression of MHC and costimulatory molecules, was induced by incubation with lipopolysaccharide, agonistic CD40 antibody, or specific CD4+ T helper (Th) cells. Activated, but not immature, D1 cells efficiently primed alloreactive T cell responses in vitro. Similarly, priming of CTL immunity in vivo in CD4-depleted mice was only observed if these mice were immunized with activated D1 cells. This study provides formal evidence that activation of DCs, induced by Th-independent as well as Th-dependent stimuli, is essential for efficient induction of CTL responses.

Key Words: dendritic cells • CD40 ligation • lipopolysaccharide • Th cells • cytotoxic T lymphocyte induction

© 2000 The Rockefeller University Press


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