Original Article

Enterocyte Expression of Interleukin 7 Induces Development of T Cells and Peyer's Patches

Correspondence to: Lynn Puddington, Dept. of Medicine, MC-1310, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030. Tel:860-679-4655 Fax:860-679-1287 E-mail:lpudding{at}panda.uchc.edu.

The intestinal mucosa is suggested to support extrathymic T cell development, particularly for T cell receptor (TCR)- intraepithelial lymphocytes (IELs). TCR- cell development requires interleukin (IL)-7; IL-7^-/- or IL-7 receptor^-/- mice lack TCR- cells. Using the intestinal fatty acid binding protein (iFABP) promoter, we reinstated expression of IL-7 to mature enterocytes of IL-7^-/- mice (iFABP-IL7). In iFABP-IL7 mice, TCR- IELs were restored, as were cryptopatches and Peyer's patches. TCR- cells remained absent from all other tissues. Likewise, T cell development in thymus and B cell maturation in the bone marrow and spleen retained the IL-7^-/- phenotype. Thus, IL-7 expression by enterocytes was sufficient for extrathymic development of TCR- cells in situ within the intestinal epithelium and was crucial for organization of mucosal lymphoid tissue.

Key Words: immunology, cell differentiation, morphogenesis, germinal center

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