The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
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Published online 1 May 2000.
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© The Rockefeller University Press, 0022-1007/2000/5/1569/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 9, May 1, 2000 1569-1580

Original Article

Enterocyte Expression of Interleukin 7 Induces Development of {gamma}{delta} T Cells and Peyer's Patches

Karen Lakya, Leo Lefrançoisa, Elizabeth G. Lingenhelda, Hiromichi Ishikawab, Julia M. Lewisc, Sara Olsona, Kenji Suzukib, Robert E. Tigelaarc, and Lynn Puddingtona

a Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030-1310
b Department of Microbiology, Keio University School of Medicine, Tokyo 160-8582, Japan
c Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06520-8059
Dept. of Medicine, MC-1310, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030.860-679-1287860-679-4655

lpudding{at}panda.uchc.edu

The intestinal mucosa is suggested to support extrathymic T cell development, particularly for T cell receptor (TCR)-{gamma}{delta} intraepithelial lymphocytes (IELs). TCR-{gamma}{delta} cell development requires interleukin (IL)-7; IL-7–/– or IL-7 receptor–/– mice lack TCR-{gamma}{delta} cells. Using the intestinal fatty acid binding protein (iFABP) promoter, we reinstated expression of IL-7 to mature enterocytes of IL-7–/– mice (iFABP-IL7). In iFABP-IL7 mice, TCR-{gamma}{delta} IELs were restored, as were cryptopatches and Peyer's patches. TCR-{gamma}{delta} cells remained absent from all other tissues. Likewise, T cell development in thymus and B cell maturation in the bone marrow and spleen retained the IL-7–/– phenotype. Thus, IL-7 expression by enterocytes was sufficient for extrathymic development of TCR-{gamma}{delta} cells in situ within the intestinal epithelium and was crucial for organization of mucosal lymphoid tissue.

Key Words: immunology • cell differentiation • morphogenesis • germinal center

Abbreviations used in this paper: DETC, dendritic epidermal T cell; IELs, intraepithelial lymphocytes; iFABP, intestinal fatty acid binding protein; LP, lamina propria; PNA, peanut agglutinin; SCF, stem cell factor.

© 2000 The Rockefeller University Press


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