The Journal of Experimental Medicine
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Published online 6 March 2000.
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© The Rockefeller University Press, 0022-1007/2000/3/823/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 5, March 6, 2000 823-834

Original Article

Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph

Tuula Arstilaa, T. Petteri Arstilab, Sébastien Calbob, Françoise Selza, Michèle Malassis-Serisa, Pierre Vassallic, Philippe Kourilskyb, and Delphine Guy-Granda,b

a Institut National de la Santé et de la Recherche Médicale (INSERM) U429, Hôpital Necker-Enfants Malades, 75743 Paris Cedex 15, France
b Unité de Biologie Moléculaire du Gène, INSERM U277 and Institut Pasteur, 75724 Paris Cedex 15, France
c Département de Pathologie, Centre Médical Universitaire, CH-1211 Geneva 4, Switzerland
Unité de Biologie Moléculaire du Gène, INSERM U277 and Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.33-1-45-68-85-4833-1-40-61-32-09

guygrand{at}pasteur.fr

Murine gut intraepithelial (IEL) T cell receptor (TCR)-{alpha}/β1 lymphocytes bearing CD8{alpha}/β or CD8{alpha}/{alpha} coreceptors have been shown previously to express different oligoclonal TCR β chain repertoires in the same mouse, in agreement with other evidence indicating that these two populations belong to different ontogenic lineages, with only CD8{alpha}/β1 IELs being fully thymus dependent. CD8{alpha}/β1, but not CD8{alpha}/{alpha}1, T lymphocytes are also present in the lamina propria. Here, we show that CD8{alpha}/β+ lymphocytes from the lamina propria and the epithelium are both oligoclonal, and that they share the same TCR-β clonotypes in the same mouse, as is also the case for CD4+ T cells. Furthermore, identical T cell clones were detected among CD8{alpha}/β1 IELs and CD8{alpha}/β1 blasts circulating into the thoracic duct (TD) lymph of the same mouse, whereas TD small lymphocytes are polyclonal. These findings must be considered in light of previous observations showing that T blasts, but not small T lymphocytes, circulating in the TD lymph have the capacity of homing into the gut epithelium and lamina propria. These combined observations have interesting implications for our understanding of the recirculation of gut thymus-dependent lymphocytes and their precursors, and of the events leading up to the selection of their restricted TCR repertoire.

Key Words: gut lymphocyte • TCR-β repertoire • lymphocyte circulation • thoracic duct lymphocyte

Abbreviations used in this paper: BrdU, 5-bromo-2'-deoxyuridine; IEL, intraepithelial lymphocyte; LPL, lamina propria lymphocyte; LT, lymphotoxin; TD, thoracic duct.

© 2000 The Rockefeller University Press


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