Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 98K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Obst, R. Articles by Rammensee, H.-G. Search for Related Content PubMed PubMed Citation Articles by Obst, R. Articles by Rammensee, H.-G. Social Bookmarking                            What's this?

Original Article

Reinhard Obst^a, Nikolai Netuschil^a, Karsten Klopfer^a, Stefan Stevanovi^a, and Hans-Georg Rammensee^a

^a Department of Immunology, Institute for Cell Biology, University of Tübingen, D-72076 Tübingen, Germany
Department of Immunology, Institute for Cell Biology, Eberhard-Karls-University Tübingen, Auf der Morgenstelle 15, D-72076 Tübingen, Germany.49-7071-29-56-5349-7071-298-09-91

rammensee{at}uni-tuebingen.de

By analyzing T cell responses against foreign major histocompatibility complex (MHC) molecules loaded with peptide libraries and defined self- and viral peptides, we demonstrate a profound influence of self-MHC molecules on the repertoire of alloreactive T cells: the closer the foreign MHC molecule is related to the T cell's MHC, the higher is the proportion of peptide-specific, alloreactive ("allorestricted") T cells versus T cells recognizing the foreign MHC molecule without regard to the peptide in the groove. Thus, the peptide repertoire of alloreactive T cells must be influenced by self-MHC molecules during positive or negative thymic selection or peripheral survival, much like the repertoire of the self-restricted T cells. In consequence, allorestricted, peptide-specific T cells (that are of interest for clinical applications) are easier to obtain if T cells and target cells express related MHC molecules.

Key Words: peptide library • T cell repertoire • molecular basis of alloreactivity • limiting dilution • positive selection

Abbreviations used in this paper: B6, C57BL/6; K^bL or D^bL, K^b or D^b binding peptide library, respectively; K^b_(self), mixture of 10 K^b binding self-peptides; TAP, transporter associated with antigen processing; VSV, vesicular stomatitis virus.

© 2000 The Rockefeller University Press

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS