The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/2000/2/743/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 4, February 21, 2000 743-748

Brief Definitive Report

The Role of Aquaporins in Dendritic Cell Macropinocytosis

Annegret de Baeya and Antonio Lanzavecchiaa

a From the Basel Institute for Immunology, CH-4005 Basel, Switzerland
Basel Institute for Immunology, Grenzacherstr. 487, CH-4005 Basel, Switzerland.41-61-605-13-6441-61-605-13-26

lanzavecchia{at}bii.ch

Immature dendritic cells (DCs) constitutively take up large volumes of fluid by macropinocytosis and concentrate the macrosolutes in the endocytic compartment. This concentration mechanism that is the basis of their high capacity to present soluble antigens requires that DCs be capable of rapidly exchanging water across their membranes. We report that two members of the aquaporin family, AQP3 and AQP7, are expressed in immature DCs and are downregulated after maturation. Treatment of DCs with p-chloromercuribenzenesulphonate (pCMBS), a mercuric drug that blocks aquaporins, inhibited uptake and concentration of macrosolutes taken up by fluid phase endocytosis and led to dramatic cell swelling. In contrast, pCMBS did not affect receptor-mediated endocytosis via the mannose receptor. These findings indicate that aquaporins represent essential elements of a volume control mechanism that allows DCs to concentrate macrosolutes taken up via macropinocytosis.

Key Words: dendritic cells • fluid phase endocytosis • aquaporins • antigen uptake • volume control

© 2000 The Rockefeller University Press


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