Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 116K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Josien, R. Articles by Choi, Y. Search for Related Content PubMed PubMed Citation Articles by Josien, R. Articles by Choi, Y. Social Bookmarking                            What's this?

Original Article

^a From the Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, New York 10021
^b From the Laboratory of Immunology, The Rockefeller University, New York, New York 10021
^c From the Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021
^d Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455
Laboratory of Cellular Physiology and Immunology, The Rockefeller University, 1230 York Ave., 405 Bronk Bldg., New York, NY 10021.212-327-8875212-327-8106

steinma{at}rockvax.rockefeller.edu

Mature dendritic cells (DCs) are powerful antigen presenting cells that have the unique capacity to migrate to the T cell zone of draining lymph nodes after subcutaneous injection. Here we report that treatment of antigen-pulsed mature DCs with tumor necrosis factor (TNF)-related activation-induced cytokine (TRANCE), a TNF family member, before immunization enhances their adjuvant capacity and elicits improved T cell priming in vivo, such that both primary and memory T cell immune responses are enhanced. By enumerating migratory DCs in the draining lymph nodes and by studying their function in stimulating naive T cells, we show that one of the underlying mechanisms for enhanced T cell responses is an increase in the number of ex vivo antigen-pulsed DCs that are found in the T cell areas of lymph nodes. These results suggest that the longevity and abundance of mature DCs at the site of T cell priming influence the strength of the DC-initiated T cell immunity in situ. Our findings have the potential to improve DC-based immunotherapy; i.e., the active immunization of humans with autologous DCs that have been pulsed with clinically significant antigens ex vivo.

Key Words: TRANCE • dendritic cells • T cell • immunization

Y. Choi, Laboratory of Immunology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: 212-327-7441; Fax: 212-327-7319; E-mail: choi@rockvax.rockefeller.edu

Abbreviations used in this paper: DC, dendritic cell; DTH, delayed-type hypersensitivity; PPD, purified protein derivative; TRANCE, TNF-related activation-induced cytokine.

© 2000 The Rockefeller University Press

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS