Original Article

Critical Role for CD8 in T Cell Receptor Binding and Activation by Peptide/Major Histocompatibility Complex Multimers

Recent data using MHC/peptide tetramers and dimers suggests that the T cell coreceptors, CD4 and CD8, although important for T cell activation, do not play a direct role in facilitating T cell receptor (TCR) binding to multivalent MHC/peptide ligands. Instead, a current model proposes that coreceptors are recruited only after a stable TCR–MHC/peptide complex has already formed and signaled. In contrast, we show using multimeric class I MHC/peptide ligands that CD8 plays a critical (in some cases obligatory) role in antigen-specific TCR binding. T cell activation, measured by calcium mobilization, was induced by multimeric but not monomeric ligands and also showed CD8 dependency. Our analysis using anti-CD8 antibodies revealed that binding to different epitopes of CD8 can either block or augment TCR–MHC/peptide interaction. These effects on TCR binding to high-affinity agonist ligands were even more pronounced when binding to multimeric low-affinity ligands, including TCR antagonists, was studied. Our data have important implications for the role of CD8 in TCR binding to MHC/peptide ligands and in T cell activation. In addition, our results argue against the view that multimeric MHC/peptide ligands bind directly and solely to the TCR; rather, our data highlight a pivotal contribution of CD8 for this association.

Key Words: MHC/peptide tetramers, T cell activation, cytotoxic T lymphocyte, calcium, flow cytometry

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Chervin, A. S., Stone, J. D., Holler, P. D., Bai, A., Chen, J., Eisen, H. N., Kranz, D. M. (2009). The Impact of TCR-Binding Properties and Antigen Presentation Format on T Cell Responsiveness. J. Immunol. 183: 1166-1178 [Abstract] [Full Text]  
• Rettig, L., McNeill, L., Sarner, N., Guillaume, P., Luescher, I., Tolaini, M., Kioussis, D., Zamoyska, R. (2009). An Essential Role for the Stalk Region of CD8{beta} in the Coreceptor Function of CD8. J. Immunol. 182: 121-129 [Abstract] [Full Text]  
• Apte, S. H., Baz, A., Groves, P., Kelso, A., Kienzle, N. (2008). Interferon-{gamma} and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells. Proc. Natl. Acad. Sci. USA 105: 17475-17480 [Abstract] [Full Text]  
• Jones, L. L., Colf, L. A., Stone, J. D., Garcia, K. C., Kranz, D. M. (2008). Distinct CDR3 Conformations in TCRs Determine the Level of Cross-Reactivity for Diverse Antigens, but Not the Docking Orientation. J. Immunol. 181: 6255-6264 [Abstract] [Full Text]  
• Endo, S., Sakamoto, Y., Kobayashi, E., Nakamura, A., Takai, T. (2008). Regulation of cytotoxic T lymphocyte triggering by PIR-B on dendritic cells. Proc. Natl. Acad. Sci. USA 105: 14515-14520 [Abstract] [Full Text]  
• Andrews, N. P., Pack, C. D., Lukacher, A. E. (2008). Generation of Antiviral Major Histocompatibility Complex Class I-Restricted T Cells in the Absence of CD8 Coreceptors. J. Virol. 82: 4697-4705 [Abstract] [Full Text]  
• Gil, D., Schrum, A. G., Daniels, M. A., Palmer, E. (2008). A Role for CD8 in the Developmental Tuning of Antigen Recognition and CD3 Conformational Change. J. Immunol. 180: 3900-3909 [Abstract] [Full Text]  
• Huang, J., Edwards, L. J., Evavold, B. D., Zhu, C. (2007). Kinetics of MHC-CD8 Interaction at the T Cell Membrane. J. Immunol. 179: 7653-7662 [Abstract] [Full Text]  
• Xiao, Z., Mescher, M. F., Jameson, S. C. (2007). Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. JEM 204: 2667-2677 [Abstract] [Full Text]  
• Yang, W, Beaudoin, E., Lu, L, Du Pasquier, R., Kuroda, M., Willemsen, R., Koralnik, I., Junghans, R. (2007). Chimeric immune receptors (CIRs) specific to JC virus for immunotherapy in progressive multifocal leukoencephalopathy (PML). Int Immunol 19: 1083-1093 [Abstract] [Full Text]  
• Sandau, M. M., Winstead, C. J., Jameson, S. C. (2007). IL-15 Is Required for Sustained Lymphopenia-Driven Proliferation and Accumulation of CD8 T Cells. J. Immunol. 179: 120-125 [Abstract] [Full Text]  
• Cush, S. S., Anderson, K. M., Ravneberg, D. H., Weslow-Schmidt, J. L., Flano, E. (2007). Memory Generation and Maintenance of CD8+ T Cell Function during Viral Persistence. J. Immunol. 179: 141-153 [Abstract] [Full Text]  
• Leng, Q., Ge, Q., Nguyen, T., Eisen, H. N., Chen, J. (2007). Stage-dependent reactivity of thymocytes to self-peptide-MHC complexes. Proc. Natl. Acad. Sci. USA 104: 5038-5043 [Abstract] [Full Text]  
• Lyons, G. E., Moore, T., Brasic, N., Li, M., Roszkowski, J. J., Nishimura, M. I. (2006). Influence of Human CD8 on Antigen Recognition by T-Cell Receptor-Transduced Cells. Cancer Res. 66: 11455-11461 [Abstract] [Full Text]  
• Archbold, J. K., Macdonald, W. A., Miles, J. J., Brennan, R. M., Kjer-Nielsen, L., McCluskey, J., Burrows, S. R., Rossjohn, J. (2006). Alloreactivity between Disparate Cognate and Allogeneic pMHC-I Complexes Is the Result of Highly Focused, Peptide-dependent Structural Mimicry. J. Biol. Chem. 281: 34324-34332 [Abstract] [Full Text]  
• Guillaume, P., Baumgaertner, P., Angelov, G. S., Speiser, D., Luescher, I. F. (2006). Fluorescence-Activated Cell Sorting and Cloning of Bona Fide CD8+ CTL with Reversible MHC-Peptide and Antibody Fab' Conjugates. J. Immunol. 177: 3903-3912 [Abstract] [Full Text]  
• Neveu, B., Echasserieau, K., Hill, T., Kuus-Reichel, K., Houssaint, E., Bonneville, M., Saulquin, X. (2006). Impact of CD8-MHC class I interaction in detection and sorting efficiencies of antigen-specific T cells using MHC class I/peptide multimers: contribution of pMHC valency. Int Immunol 18: 1139-1145 [Abstract] [Full Text]  
• Kao, C., Sandau, M. M., Daniels, M. A., Jameson, S. C. (2006). The Sialyltransferase ST3Gal-I Is Not Required for Regulation of CD8-Class I MHC Binding during T Cell Development.. J. Immunol. 176: 7421-7430 [Abstract] [Full Text]  
• Angelov, G. S., Guillaume, P., Cebecauer, M., Bosshard, G., Dojcinovic, D., Baumgaertner, P., Luescher, I. F. (2006). Soluble MHC-Peptide Complexes Containing Long Rigid Linkers Abolish CTL-Mediated Cytotoxicity. J. Immunol. 176: 3356-3365 [Abstract] [Full Text]  
• Kim, M., Moon, H.-B., Kim, K., Lee, K.-Y. (2006). Antigen dose governs the shaping of CTL repertoires in vitro and in vivo. Int Immunol 18: 435-444 [Abstract] [Full Text]  
• Stone, J. D., Stern, L. J. (2006). CD8 T Cells, Like CD4 T Cells, Are Triggered by Multivalent Engagement of TCRs by MHC-Peptide Ligands but Not by Monovalent Engagement. J. Immunol. 176: 1498-1505 [Abstract] [Full Text]  
• Kao, C., Daniels, M. A., Jameson, S. C. (2005). Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation. Int Immunol 17: 1607-1617 [Abstract] [Full Text]  
• Kedzierska, K., La Gruta, N. L., Davenport, M. P., Turner, S. J., Doherty, P. C. (2005). Contribution of T cell receptor affinity to overall avidity for virus-specific CD8+ T cell responses. Proc. Natl. Acad. Sci. USA 102: 11432-11437 [Abstract] [Full Text]  
• Drake, D. R. III, Ream, R. M., Lawrence, C. W., Braciale, T. J. (2005). Transient Loss of MHC Class I Tetramer Binding after CD8+ T Cell Activation Reflects Altered T Cell Effector Function. J. Immunol. 175: 1507-1515 [Abstract] [Full Text]  
• Wooldridge, L., van den Berg, H. A., Glick, M., Gostick, E., Laugel, B., Hutchinson, S. L., Milicic, A., Brenchley, J. M., Douek, D. C., Price, D. A., Sewell, A. K. (2005). Interaction between the CD8 Coreceptor and Major Histocompatibility Complex Class I Stabilizes T Cell Receptor-Antigen Complexes at the Cell Surface. J. Biol. Chem. 280: 27491-27501 [Abstract] [Full Text]  
• Holman, P. O., Walsh, E. R., Jameson, S. C. (2005). Characterizing the Impact of CD8 Antibodies on Class I MHC Multimer Binding. J. Immunol. 174: 3986-3991 [Abstract] [Full Text]  
• Ryschich, E., Notzel, T., Hinz, U., Autschbach, F., Ferguson, J., Simon, I., Weitz, J., Frohlich, B., Klar, E., Buchler, M. W., Schmidt, J. (2005). Control of T-Cell-Mediated Immune Response by HLA Class I in Human Pancreatic Carcinoma. Clin. Cancer Res. 11: 498-504 [Abstract] [Full Text]  
• Oh, S., Perera, L. P., Burke, D. S., Waldmann, T. A., Berzofsky, J. A. (2004). IL-15/IL-15R{alpha}-mediated avidity maturation of memory CD8+ T cells. Proc. Natl. Acad. Sci. USA 101: 15154-15159 [Abstract] [Full Text]  
• Mueller, K. L., Daniels, M. A., Felthauser, A., Kao, C., Jameson, S. C., Shimizu, Y. (2004). Cutting Edge: LFA-1 Integrin-Dependent T Cell Adhesion Is Regulated by Both Ag Specificity and Sensitivity. J. Immunol. 173: 2222-2226 [Abstract] [Full Text]  
• van Oijen, M., Bins, A., Elias, S., Sein, J., Weder, P., de Gast, G., Mallo, H., Gallee, M., van Tinteren, H., Schumacher, T., Haanen, J. (2004). On the Role of Melanoma-Specific CD8+ T-Cell Immunity in Disease Progression of Advanced-Stage Melanoma Patients. Clin. Cancer Res. 10: 4754-4760 [Abstract] [Full Text]  
• Schell, T. D. (2004). In Vivo Expansion of the Residual Tumor Antigen-Specific CD8+ T Lymphocytes That Survive Negative Selection in Simian Virus 40 T-Antigen-Transgenic Mice. J. Virol. 78: 1751-1762 [Abstract] [Full Text]  
• Mintern, J. D., Maurice, M. M., Ploegh, H. L., Schott, E. (2004). Thymic Selection and Peripheral Activation of CD8 T Cells by the Same Class I MHC/Peptide Complex. J. Immunol. 172: 699-708 [Abstract] [Full Text]  
• Wooldridge, L., Hutchinson, S. L., Choi, E. M., Lissina, A., Jones, E., Mirza, F., Dunbar, P. R., Price, D. A., Cerundolo, V., Sewell, A. K. (2003). Anti-CD8 Antibodies Can Inhibit or Enhance Peptide-MHC Class I (pMHCI) Multimer Binding: This Is Paralleled by Their Effects on CTL Activation and Occurs in the Absence of an Interaction between pMHCI and CD8 on the Cell Surface. J. Immunol. 171: 6650-6660 [Abstract] [Full Text]  
• Choi, E. M.-L., Chen, J.-L., Wooldridge, L., Salio, M., Lissina, A., Lissin, N., Hermans, I. F., Silk, J. D., Mirza, F., Palmowski, M. J., Dunbar, P. R., Jakobsen, B. K., Sewell, A. K., Cerundolo, V. (2003). High Avidity Antigen-Specific CTL Identified by CD8-Independent Tetramer Staining. J. Immunol. 171: 5116-5123 [Abstract] [Full Text]  
• Kerry, S. E., Buslepp, J., Cramer, L. A., Maile, R., Hensley, L. L., Nielsen, A. I., Kavathas, P., Vilen, B. J., Collins, E. J., Frelinger, J. A. (2003). Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Affinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement. J. Immunol. 171: 4493-4503 [Abstract] [Full Text]  
• Pittet, M. J., Rubio-Godoy, V., Bioley, G., Guillaume, P., Batard, P., Speiser, D., Luescher, I., Cerottini, J.-C., Romero, P., Zippelius, A. (2003). {alpha}3 Domain Mutants of Peptide/MHC Class I Multimers Allow the Selective Isolation of High Avidity Tumor-Reactive CD8 T Cells. J. Immunol. 171: 1844-1849 [Abstract] [Full Text]  
• Wong, J. S., Wang, X., Witte, T., Nie, L., Carvou, N., Kern, P., Chang, H.-C. (2003). Stalk Region of {beta}-Chain Enhances the Coreceptor Function of CD8. J. Immunol. 171: 867-874 [Abstract] [Full Text]  
• Doucey, M.-A., Legler, D. F., Faroudi, M., Boucheron, N., Baumgaertner, P., Naeher, D., Cebecauer, M., Hudrisier, D., Ruegg, C., Palmer, E., Valitutti, S., Bron, C., Luescher, I. F. (2003). The {beta}1 and {beta}3 Integrins Promote T Cell Receptor-mediated Cytotoxic T Lymphocyte Activation. J. Biol. Chem. 278: 26983-26991 [Abstract] [Full Text]  
• Dutoit, V., Guillaume, P., Ayyoub, M., Hesdorffer, C. S., Luescher, I. F., Valmori, D. (2003). Decreased Binding of Peptides-MHC Class I (pMHC) Multimeric Complexes to CD8 Affects Their Binding Avidity for the TCR But Does Not Significantly Impact on pMHC/TCR Dissociation Rate. J. Immunol. 170: 5110-5117 [Abstract] [Full Text]  
• Curtsinger, J. M., Lins, D. C., Mescher, M. F. (2003). Signal 3 Determines Tolerance versus Full Activation of Naive CD8 T Cells: Dissociating Proliferation and Development of Effector Function. JEM 197: 1141-1151 [Abstract] [Full Text]  
• Guillaume, P., Legler, D. F., Boucheron, N., Doucey, M.-A., Cerottini, J.-C., Luescher, I. F. (2003). Soluble Major Histocompatibility Complex-Peptide Octamers with Impaired CD8 Binding Selectively Induce Fas-dependent Apoptosis. J. Biol. Chem. 278: 4500-4509 [Abstract] [Full Text]  
• Doucey, M.-A., Goffin, L., Naeher, D., Michielin, O., Baumgartner, P., Guillaume, P., Palmer, E., Luescher, I. F. (2003). CD3delta Establishes a Functional Link between the T Cell Receptor and CD8. J. Biol. Chem. 278: 3257-3264 [Abstract] [Full Text]  
• Buslepp, J., Kerry, S. E., Loftus, D., Frelinger, J. A., Appella, E., Collins, E. J. (2003). High Affinity Xenoreactive TCR:MHC Interaction Recruits CD8 in Absence of Binding to MHC. J. Immunol. 170: 373-383 [Abstract] [Full Text]  
• Ge, Q., Stone, J. D., Thompson, M. T., Cochran, J. R., Rushe, M., Eisen, H. N., Chen, J., Stern, L. J. (2002). Soluble peptide-MHC monomers cause activation of CD8+ T cells through transfer of the peptide to T cell MHC molecules. Proc. Natl. Acad. Sci. USA 99: 13729-13734 [Abstract] [Full Text]  
• Schott, E., Bertho, N., Ge, Q., Maurice, M. M., Ploegh, H. L. (2002). Class I negative CD8 T cells reveal the confounding role of peptide-transfer onto CD8 T cells stimulated with soluble H2-Kb molecules. Proc. Natl. Acad. Sci. USA 99: 13735-13740 [Abstract] [Full Text]  
• Wang, B., Sharma, A., Maile, R., Saad, M., Collins, E. J., Frelinger, J. A. (2002). Peptidic Termini Play a Significant Role in TCR Recognition. J. Immunol. 169: 3137-3145 [Abstract] [Full Text]  
• Sidobre, S., Naidenko, O. V., Sim, B.-C., Gascoigne, N. R. J., Garcia, K. C., Kronenberg, M. (2002). The V{alpha}14 NKT Cell TCR Exhibits High-Affinity Binding to a Glycolipid/CD1d Complex. J. Immunol. 169: 1340-1348 [Abstract] [Full Text]  
• Dutoit, V., Rubio-Godoy, V., Pittet, M. J., Zippelius, A., Dietrich, P.-Y., Legal, F. A., Guillaume, P., Romero, P., Cerottini, J.-C., Houghten, R. A., Pinilla, C., Valmori, D. (2002). Degeneracy of Antigen Recognition as the Molecular Basis for the High Frequency of Naive A2/Melan-A Peptide Multimer+ CD8+ T Cells in Humans. JEM 196: 207-216 [Abstract] [Full Text]  
• Freese, A., Zavazava, N. (2002). HLA-B7 beta -pleated sheet-derived synthetic peptides are immunodominant T-cell epitopes regulating alloresponses. Blood 99: 3286-3292 [Abstract] [Full Text]  
• Dutoit, V., Rubio-Godoy, V., Doucey, M.-A., Batard, P., Lienard, D., Rimoldi, D., Speiser, D., Guillaume, P., Cerottini, J.-C., Romero, P., Valmori, D. (2002). Functional Avidity of Tumor Antigen-Specific CTL Recognition Directly Correlates with the Stability of MHC/Peptide Multimer Binding to TCR. J. Immunol. 168: 1167-1171 [Abstract] [Full Text]  
• Miconnet, I., Koenig, S., Speiser, D., Krieg, A., Guillaume, P., Cerottini, J.-C., Romero, P. (2002). CpG Are Efficient Adjuvants for Specific CTL Induction Against Tumor Antigen-Derived Peptide. J. Immunol. 168: 1212-1218 [Abstract] [Full Text]  
• Campanelli, R., Palermo, B., Garbelli, S., Mantovani, S., Lucchi, P., Necker, A., Lantelme, E., Giachino, C. (2002). Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation. Int Immunol 14: 39-44 [Abstract] [Full Text]  
• Arcaro, A., Gregoire, C., Bakker, T. R., Baldi, L., Jordan, M., Goffin, L., Boucheron, N., Wurm, F., van der Merwe, P. A., Malissen, B., Luescher, I. F. (2001). CD8{beta} Endows CD8 with Efficient Coreceptor Function by Coupling T Cell Receptor/CD3 to Raft-associated CD8/p56lck Complexes. JEM 194: 1485-1495 [Abstract] [Full Text]  
• Bullock, T. N. J., Mullins, D. W., Colella, T. A., Engelhard, V. H. (2001). Manipulation of Avidity to Improve Effectiveness of Adoptively Transferred CD8+ T Cells for Melanoma Immunotherapy in Human MHC Class I-Transgenic Mice. J. Immunol. 167: 5824-5831 [Abstract] [Full Text]  
• Holler, P. D., Lim, A. R., Cho, B. K., Rund, L. A., Kranz, D. M. (2001). CD8- T Cell Transfectants that Express a High Affinity T Cell Receptor Exhibit Enhanced Peptide-dependent Activation. JEM 194: 1043-1052 [Abstract] [Full Text]  
• O'Herrin, S. M., Slansky, J. E., Tang, Q., Markiewicz, M. A., Gajewski, T. F., Pardoll, D. M., Schneck, J. P., Bluestone, J. A. (2001). Antigen-Specific Blockade of T Cells In Vivo Using Dimeric MHC Peptide. J. Immunol. 167: 2555-2560 [Abstract] [Full Text]  
• Tham, E. L., Mescher, M. F. (2001). Signaling Alterations in Activation-Induced Nonresponsive CD8 T Cells. J. Immunol. 167: 2040-2048 [Abstract] [Full Text]  
• Tsujimura, K., Obata, Y., Matsudaira, Y., Ozeki, S., Yoshikawa, K., Saga, S., Takahashi, T. (2001). The Binding of Thymus Leukemia (TL) Antigen Tetramers to Normal Intestinal Intraepithelial Lymphocytes and Thymocytes. J. Immunol. 167: 759-764 [Abstract] [Full Text]  
• Block, M. S., Johnson, A. J., Mendez-Fernandez, Y., Pease, L. R. (2001). Monomeric Class I Molecules Mediate TCR/CD3{{epsilon}}/CD8 Interaction on the Surface of T Cells. J. Immunol. 167: 821-826 [Abstract] [Full Text]  
• Tindle, R. W., Herd, K., Doan, T., Bryson, G., Leggatt, G. R., Lambert, P., Frazer, I. H., Street, M. (2001). Nonspecific Down-Regulation of CD8+ T-Cell Responses in Mice Expressing Human Papillomavirus Type 16 E7 Oncoprotein from the Keratin-14 Promoter. J. Virol. 75: 5985-5997 [Abstract] [Full Text]  
• Denkberg, G., Cohen, C. J., Reiter, Y. (2001). Critical Role for CD8 in Binding of MHC Tetramers to TCR: CD8 Antibodies Block Specific Binding of Human Tumor- Specific MHC-Peptide Tetramers to TCR. J. Immunol. 167: 270-276 [Abstract] [Full Text]  
• Drake, D. R. III, Braciale, T. J. (2001). Cutting Edge: Lipid Raft Integrity Affects the Efficiency of MHC Class I Tetramer Binding and Cell Surface TCR Arrangement on CD8+ T Cells. J. Immunol. 166: 7009-7013 [Abstract] [Full Text]  
• Derby, M. A., Wang, J., Margulies, D. H., Berzofsky, J. A. (2001). Two intermediate-avidity cytotoxic T lymphocyte clones with a disparity between functional avidity and MHC tetramer staining. Int Immunol 13: 817-824 [Abstract] [Full Text]  
• Hudrisier, D., Riond, J., Mazarguil, H., Gairin, J. E., Joly, E. (2001). Cutting Edge: CTLs Rapidly Capture Membrane Fragments from Target Cells in a TCR Signaling-Dependent Manner. J. Immunol. 166: 3645-3649 [Abstract] [Full Text]  
• Hernandez, J., Ko, A., Sherman, L. A. (2001). CTLA-4 Blockade Enhances the CTL Responses to the p53 Self-Tumor Antigen. J. Immunol. 166: 3908-3914 [Abstract] [Full Text]  
• Lohwasser, S., Kubota, A., Salcedo, M., Lian, R. H., Takei1, F. (2001). The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes. Int Immunol 13: 321-327 [Abstract] [Full Text]  
• Cho, B. K., Lian, K.-C., Lee, P., Brunmark, A., McKinley, C., Chen, J., Kranz, D. M., Eisen, H. N. (2001). Differences in antigen recognition and cytolytic activity of CD8+ and CD8- T cells that express the same antigen-specific receptor. Proc. Natl. Acad. Sci. USA 98: 1723-1727 [Abstract] [Full Text]  
• Ge, Q., Rao, V. P., Cho, B. K., Eisen, H. N., Chen, J. (2001). Dependence of lymphopenia-induced T cell proliferation on the abundance of peptide/ MHC epitopes and strength of their interaction with T cell receptors. Proc. Natl. Acad. Sci. USA 98: 1728-1733 [Abstract] [Full Text]  
• Reichstetter, S., Ettinger, R. A., Liu, A. W., Gebe, J. A., Nepom, G. T., Kwok, W. W. (2000). Distinct T Cell Interactions with HLA Class II Tetramers Characterize a Spectrum of TCR Affinities in the Human Antigen-Specific T Cell Response. J. Immunol. 165: 6994-6998 [Abstract] [Full Text]  
• Arcaro, A., Gregoire, C., Boucheron, N., Stotz, S., Palmer, E., Malissen, B., Luescher, I. F. (2000). Essential Role of CD8 Palmitoylation in CD8 Coreceptor Function. J. Immunol. 165: 2068-2076 [Abstract] [Full Text]  
• Skinner, P. J., Daniels, M. A., Schmidt, C. S., Jameson, S. C., Haase, A. T. (2000). Cutting Edge: In Situ Tetramer Staining of Antigen-Specific T Cells in Tissues. J. Immunol. 165: 613-617 [Abstract] [Full Text]  
• Purbhoo, M. A., Boulter, J. M., Price, D. A., Vuidepot, A.-L., Hourigan, C. S., Dunbar, P. R., Olson, K., Dawson, S. J., Phillips, R. E., Jakobsen, B. K., Bell, J. I., Sewell, A. K. (2001). The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor zeta Chain. J. Biol. Chem. 276: 32786-32792 [Abstract] [Full Text]  
• Germain, R. N. (2001). The T Cell Receptor for Antigen: Signaling and Ligand Discrimination. J. Biol. Chem. 276: 35223-35226 [Full Text]  
• Luz, J. G., Huang, M., Garcia, K. C., Rudolph, M. G., Apostolopoulos, V., Teyton, L., Wilson, I. A. (2002). Structural Comparison of Allogeneic and Syngeneic T Cell Receptor-Peptide-Major Histocompatibility Complex Complexes: A Buried Alloreactive Mutation Subtly Alters Peptide Presentation Substantially Increasing V{beta} Interactions. JEM 195: 1175-1186 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS