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Original Article

Fc Receptor (Cd89) Mediates the Development of Immunoglobulin a (Iga) Nephropathy (Berger's Disease)

Pierre Launay^a, Béatrice Grossetête^a, Michelle Arcos-Fajardo^a, Emmanuelle Gaudin^a, Sonia P. Torres^a, Lucie Beaudoin^a, Natacha Patey-Mariaud de Serre^b, Agnès Lehuen^a, and Renato C. Monteiro^a

^a Institut National de la Santé et de la Recherche Médicale U25, Necker Hospital, Paris 75743, France
^b Department of Pathology, Necker Hospital, Paris 75743, France
INSERM U25, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France.33-1-43-06-23-8833-1-44-49-53-66

monteiro{at}necker.fr

The pathogenesis of immunoglobulin A (IgA) nephropathy (IgAN), the most prevalent form of glomerulonephritis worldwide, involves circulating macromolecular IgA1 complexes. However, the molecular mechanism(s) of the disease remain poorly understood. We report here the presence of circulating soluble FcR (CD89)-IgA complexes in patients with IgAN. Soluble CD89 was identified as a glycoprotein with a 24-kD backbone that corresponds to the expected size of CD89 extracellular domains. To demonstrate their pathogenic role, we generated transgenic (Tg) mice expressing human CD89 on macrophage/monocytes, as no CD89 homologue is found in mice. These mice spontaneously developed massive mesangial IgA deposition, glomerular and interstitial macrophage infiltration, mesangial matrix expansion, hematuria, and mild proteinuria. The molecular mechanism was shown to involve soluble CD89 released after interaction with IgA. This release was independent of CD89 association with the FcR chain. The disease was induced in recombination activating gene (RAG)2^–/– mice by injection of serum from Tg mice, and in severe combined immunodeficiency (SCID)-Tg mice by injection of patients' IgA. Depletion of soluble CD89 from serum abolished this effect. These results reveal the key role of soluble CD89 in the pathogenesis of IgAN and provide an in vivo model that will be useful for developing new treatments.

Key Words: IgA nephropathy • IgA • Fc receptor • monocytes • transgenic mice

Abbreviations used in this paper: ALC, alcoholic liver cirrhosis; AP, alkaline phosphatase; BUN, blood urea nitrogen; FcR, receptor for Ig Fc fragment; HRP, horseradish peroxidase; IgAN, IgA nephropathy; IC, immune complex; MCD, minimal change disease; MSA, mouse serum albumin; NOD, nonobese diabetic; PEG, polyethylene glycol; RAG, recombination activating gene; Tg, transgenic; WT, wild-type.

© 2000 The Rockefeller University Press

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