The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/11/1257/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 9, November 1, 1999 1257-1262

Original Article

Irradiation-Mediated Rescue of T Cell–Specific V(D)j Recombination and Thymocyte Differentiation in Severe Combined Immunodeficient Mice by Bone Marrow Cells

Chiyu Wanga, Molly A. Boguea, Jonathan M. Levitta, and David B. Rothb

a Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030
b Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Baylor College of Medicine, Dept. of Immunology, M929/DeBakey Bldg., One Baylor Plaza, Houston, TX 77030-3498.713-798-3033713-798-8145

davidbr{at}bcm.tmc.edu

In SCID (severe combined immunodeficient) mice, proper assembly of immunoglobulin and T cell receptor (TCR) genes is blocked by defective V(D)J recombination so that B and T lymphocyte differentiation is arrested at an early precursor stage. Treating the mice with gamma irradiation rescues V(D)J rearrangement at multiple TCR loci, promotes limited thymocyte differentiation, and induces thymic lymphomas. These effects are not observed in the B cell lineage. Current models postulate that irradiation affects intrathymic T cell precursors. Surprisingly, we found that transfer of irradiated SCID bone marrow cells to unirradiated host animals rescues both TCR rearrangements and thymocyte differentiation. These data indicate that irradiation affects precursor cells at an earlier stage of differentiation than was previously thought and suggest new models for the mechanism of irradiation rescue.

Key Words: T cell receptors • lymphocyte differentiation • hematopoietic stem cells • TCR rearrangement • adoptive transfer

1used in this paper: DN, double negative; DP, double positive; SP, single positive

© 1999 The Rockefeller University Press


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