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© The Rockefeller University Press, 0022-1007/1999/10/1123/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 8, October 18, 1999 1123-1134

Original Article

In Vivo–Activated Cd4 T Cells Upregulate Cxc Chemokine Receptor 5 and Reprogram Their Response to Lymphoid Chemokines

K. Mark Ansela, Louise J. McHeyzer-Williamsb, Vu N. Ngoa, Michael G. McHeyzer-Williamsb, and Jason G. Cystera

a Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143
b Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710
Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Ave., San Francisco, CA 94143-0414.415-502-8424415-502-6427

cyster{at}itsa.ucsf.edu

Migration of antigen-activated CD4 T cells to B cell areas of lymphoid tissues is important for mounting T cell–dependent antibody responses. Here we show that CXC chemokine receptor (CXCR)5, the receptor for B lymphocyte chemoattractant (BLC), is upregulated on antigen-specific CD4 T cells in vivo when animals are immunized under conditions that promote T cell migration to follicles. In situ hybridization of secondary follicles for BLC showed high expression in mantle zones and low expression in germinal centers. When tested directly ex vivo, CXCR5hi T cells exhibited a vigorous chemotactic response to BLC. At the same time, the CXCR5hi cells showed reduced responsiveness to the T zone chemokines, Epstein-Barr virus–induced molecule 1 (EBI-1) ligand chemokine (ELC) and secondary lymphoid tissue chemokine (SLC). After adoptive transfer, CXCR5hi CD4 T cells did not migrate to follicles, indicating that additional changes may occur after immunization that help direct T cells to follicles. To further explore whether T cells could acquire an intrinsic ability to migrate to follicles, CD4–CD8– double negative (DN) T cells from MRL-lpr mice were studied. These T cells normally accumulate within follicles of MRL-lpr mice. Upon transfer to wild-type recipients, DN T cells migrated to follicle proximal regions in all secondary lymphoid tissues. Taken together, our findings indicate that reprogramming of responsiveness to constitutively expressed lymphoid tissue chemokines plays an important role in T cell migration to the B cell compartment of lymphoid tissues.

Key Words: chemokine • CXCR5 • ELC • follicle • T lymphocyte

1used in this paper: B6, C57BL/6; BLC, B lymphocyte chemoattractant; BLR, Burkitt's lymphoma receptor; CFSE, 5- (and 6-) carboxyfluorescein succinimidyl ester; CXCR, CXC chemokine receptor; DC, dendritic cell; DN, double negative; EBI-1, EBV-induced molecule 1; ELC, EBI-1 ligand chemokine; GC, germinal center; MIP, macrophage inflammatory protein; PCC, pigeon cytochrome c; SDF, stromal cell–derived factor; SLC, secondary lymphoid tissue chemokine

© 1999 The Rockefeller University Press


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