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Original Article

^a Department of Microbiology and Immunology and the Molecular Biology Institute, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
^b Department of Microbiology and Molecular Genetics, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
^c Division of Dermatology, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
^d Division of Digestive Diseases, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
^e La Jolla Institute for Allergy and Immunology, San Diego, California 92121
^f Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma 370-12, Japan
La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr., San Diego, CA 92121.858-678-4595858-678-4540

mitch{at}liai.org

We have purified soluble mouse and human CD1d molecules to assess the structural requirements for lipid antigen presentation by CD1. Plate-bound CD1d molecules from either species can present the glycolipid -galactosyl ceramide (-GalCer) to mouse natural killer T cells, formally demonstrating both the in vitro formation of antigenic complexes, and the presentation of -GalCer by these two CD1d molecules. Using surface plasmon resonance, we show that at neutral pH, mouse CD1 and human CD1d bind to immobilized -GalCer, unlike human CD1b, which requires acidic pH for lipid antigen binding. The CD1d molecules can also bind both to the nonantigenic β-GalCer and to phosphatidylethanolamine, indicating that diverse lipids can bind to CD1d. These studies provide the first quantitative analysis of monomeric lipid antigen–CD1 interactions, and they demonstrate that the orientation of the galactose, or even the nature of the polar head group, are likely to be more important for T cell receptor contact than CD1d binding.

Key Words: antigen presentation • CD1 • glycolipid • binding assay

© 1999 The Rockefeller University Press

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This article has been cited by other articles:

• MacDonald, H. R. (2000). Cd1d-Glycolipid Tetramers: A New Tool to Monitor Natural Killer T Cells in Health and Disease. JEM 192: f15-f20 [Full Text]  

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