A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein

doi:10.1084/jem.190.6.851

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© The Rockefeller University Press, 0022-1007/1999/9/851/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 6, September 20, 1999 851-860

Original Article

A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein

Claude Berney^a, Suzanne Herren^a, Christine A. Power^a, Siamon Gordon^b, Luisa Martinez-Pomares^b, and Marie H. Kosco-Vilbois^a

^a Serono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland
^b Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
Serono Pharmaceutical Research Institute, 14, chemin des Aulx, CH-1228 Geneva, Switzerland.41-22-794-696541-22-706-9708

marie.kosco-vilbois{at}serono.com

Dendritic cells (DCs) are known to activate naive T cells tobecome effective helper cells. In addition, recent evidencesuggests that DCs may influence naive B cells during the initialpriming of antibody responses. In this study, using three-colorconfocal microscopy and three-dimensional immunohistograms,we have observed that in the first few days after a primaryimmunization, cells with dendritic morphology progressivelylocalize within primary B cell follicles. These cells were identifiedby their ability to bind a fusion protein consisting of theterminal cysteine-rich portion of the mouse mannose receptorand the Fc portion of human immunoglobulin (Ig)G1 (CR-Fc). Insitu, these CR-Fc binding cells express major histocompatibilitycomplex class II, sialoadhesin, and CD11c and are negative forother markers identifying the myeloid DC lineage, such as (CD11b),macrophages (F4/80), follicular DCs (FDC-M2), B cells (B220),and T cells (CD4). Using CR-Fc binding capacity and flow cytometry,the cells were purified from the draining lymph nodes of mice24 h after immunization. When injected into naive mice, thesecells were able to prime T cells as well as induce productionof antigen-specific IgM and IgG1. Furthermore, they producedsignificantly more of the lymphocyte chemoattractant, macrophageinflammatory protein (MIP)-1 ${alpha}$ , than isolated interdigitatingcells. Taken together, these results provide evidence that asubset of DCs enters primary follicles, armed with the capacityto attract and provide antigenic stimulation for T and B lymphocytes.

Key Words: dendritic cells • mannose receptor • antibody production • antigen presentation • chemokines

1used in this paper: BM-DC, bone marrow–derived DC; CR,cysteine-rich; 3D, three-dimension; DC, dendritic cell; FDC,follicular DC; MFI, mean fluorescence intensity; MIP, macrophageinflammatory protein; RANTES, regulated upon activation, normalT cell expressed and secreted

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