The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/9/803/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 6, September 20, 1999 803-814

Original Article

The Glycoprotein Ib-IX-V Complex Is a Platelet Counterreceptor for P-Selectin

Gabriel M. Romoa, Jing-Fei Donga, Alicia J. Schadec, Elizabeth E. Gardinerd, Geoffrey S. Kansase, Chester Q. Lia, Larry V. McIntirec, Michael C. Berndta,d, and José A. Lópeza,b

a Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical Center, Houston, Texas 77030
b Department of Molecular and Human Genetics, Baylor College of Medicine and Veterans Affairs Medical Center, Houston, Texas 77030
c Cox Laboratory for Bioengineering, Rice University, Houston, Texas 77251
d Baker Medical Research Institute, Melbourne, Australia 3181
e Department of Microbiology/Immunology, Northwestern University Medical School, Chicago, Illinois 60611
Veterans Affairs Medical Center, Hematology/Oncology (111H), 2002 Holcombe Blvd., Houston, TX 77030.713-794-7578713-794-7088

josel{at}bcm.tmc.edu

We have identified platelet glycoprotein (GP) Ib{alpha} as a counterreceptor for P-selectin. GP Ib{alpha} is a component of the GP Ib-IX-V complex, which mediates platelet adhesion to subendothelium at sites of injury. Cells expressing P-selectin adhered to immobilized GP Ib{alpha}, and GP Ib{alpha}–expressing cells adhered to and rolled on P-selectin and on histamine-stimulated endothelium in a P-selectin–dependent manner. In like manner, platelets rolled on activated endothelium, a phenomenon inhibited by antibodies to both P-selectin and GP Ib{alpha}. Unlike the P-selectin interaction with its leukocyte ligand, PSGL-1 (P-selectin glycoprotein ligand 1), the interaction with GP Ib{alpha} required neither calcium nor carbohydrate core-2 branching or {alpha}(1,3)-fucosylation. The interaction was inhibited by sulfated proteoglycans and by antibodies against GP Ib{alpha}, including one directed at a tyrosine-sulfated region of the polypeptide. Thus, the GP Ib-IX-V complex mediates platelet attachment to both subendothelium and activated endothelium.

Key Words: platelet adhesion • endothelium • platelet glycoproteins • selectins • PSGL-1

1used in this paper: ANOVA, analysis of variance; CHO, Chinese hamster ovary; FucT, fucosyl transferase; GP, glycoprotein; HUVECs, human umbilical vein endothelial cells; PSGL-1, P-selectin glycoprotein ligand 1

© 1999 The Rockefeller University Press


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