The Glycoprotein Ib-IX-V Complex Is a Platelet Counterreceptor for P-Selectin

doi:10.1084/jem.190.6.803

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© The Rockefeller University Press, 0022-1007/1999/9/803/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 6, September 20, 1999 803-814

Original Article

The Glycoprotein Ib-IX-V Complex Is a Platelet Counterreceptor for P-Selectin

Gabriel M. Romo^a, Jing-Fei Dong^a, Alicia J. Schade^c, Elizabeth E. Gardiner^d, Geoffrey S. Kansas^e, Chester Q. Li^a, Larry V. McIntire^c, Michael C. Berndt^a^,d, and José A. López^a^,b

^a Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical Center, Houston, Texas 77030
^b Department of Molecular and Human Genetics, Baylor College of Medicine and Veterans Affairs Medical Center, Houston, Texas 77030
^c Cox Laboratory for Bioengineering, Rice University, Houston, Texas 77251
^d Baker Medical Research Institute, Melbourne, Australia 3181
^e Department of Microbiology/Immunology, Northwestern University Medical School, Chicago, Illinois 60611
Veterans Affairs Medical Center, Hematology/Oncology (111H), 2002 Holcombe Blvd., Houston, TX 77030.713-794-7578713-794-7088

josel{at}bcm.tmc.edu

We have identified platelet glycoprotein (GP) Ib ${alpha}$ as a counterreceptorfor P-selectin. GP Ib ${alpha}$ is a component of the GP Ib-IX-V complex,which mediates platelet adhesion to subendothelium at sitesof injury. Cells expressing P-selectin adhered to immobilizedGP Ib ${alpha}$ , and GP Ib ${alpha}$ –expressing cells adhered to and rolledon P-selectin and on histamine-stimulated endothelium in a P-selectin–dependentmanner. In like manner, platelets rolled on activated endothelium,a phenomenon inhibited by antibodies to both P-selectin andGP Ib ${alpha}$ . Unlike the P-selectin interaction with its leukocyteligand, PSGL-1 (P-selectin glycoprotein ligand 1), the interactionwith GP Ib ${alpha}$ required neither calcium nor carbohydrate core-2branching or ${alpha}$ (1,3)-fucosylation. The interaction was inhibitedby sulfated proteoglycans and by antibodies against GP Ib ${alpha}$ , includingone directed at a tyrosine-sulfated region of the polypeptide.Thus, the GP Ib-IX-V complex mediates platelet attachment toboth subendothelium and activated endothelium.

Key Words: platelet adhesion • endothelium • platelet glycoproteins • selectins • PSGL-1

1used in this paper: ANOVA, analysis of variance; CHO, Chinesehamster ovary; FucT, fucosyl transferase; GP, glycoprotein;HUVECs, human umbilical vein endothelial cells; PSGL-1, P-selectinglycoprotein ligand 1

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