The Journal of Experimental Medicine
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J. Exp. Med., Volume 190, Number 4, August 16, 1999 585-590
Copyright © 1999 by The Rockefeller University Press.

The Major Receptor for C-Reactive Protein on Leukocytes Is Fc{gamma} Receptor II

Dwaipayan Bharadwaja, Mary-Pat Steina, Michael Volzera, Carolyn Moldb, and Terry W. Du Closa,c
a From the Department of Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87108
b From the Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87108
c Veterans Affairs Medical Center, Albuquerque, New Mexico 87108

Correspondence to: Terry W. Du Clos, VA Medical Center, Research Service 151, 1501 San Pedro S.E., Albuquerque, NM 87108. Tel:505-256-5717 Fax:505-256-2794 E-mail:tduclos{at}unm.edu.

C-reactive protein (CRP) is an acute phase serum protein that shares several functions with immunoglobulin (Ig)G including complement activation and binding to receptors on monocytes and neutrophils. The identity of the receptor for CRP has been the target of extensive research. We previously determined that CRP binds to the high affinity receptor for IgG, Fc{gamma}RI (CD64). However, this interaction could not account for the majority of binding of CRP to neutrophils or monocytic cells. We now determine that CRP also interacts with Fc{gamma}RIIa (CD32), the low affinity receptor for IgG on monocytes and neutrophils. COS-7 cells were transfected with a construct containing the human Fc{gamma}RIIA cDNA. CRP binding and the presence of CD32 were detected by mAb and analyzed by two-color flow cytometry. Cells expressing CD32 bound CRP in a dose-dependent and saturable manner consistent with receptor binding. CRP bound to transfectants and K-562 cells with similar kinetics, and in both cases binding was completely inhibited by aggregated IgG. On monocytic cell lines, treatment with Bt2cAMP increased Fc{gamma}RII expression and enhanced CRP binding. CRP also specifically precipitated Fc{gamma}RI and Fc{gamma}RII from the monocytic cell line, THP-1. It is suggested that the major receptor for CRP on phagocytic cells is Fc{gamma}RII.

Key Words: C-reactive protein, Fc receptors, Fc{gamma}RII, receptors, immunoglobulin G


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