The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 103K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bender, J.
Right arrow Articles by Marrack, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bender, J.
Right arrow Articles by Marrack, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1007/1999/8/367/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 3, August 2, 1999 367-374

Original Article

Cd4+ T Cell Division in Irradiated Mice Requires Peptides Distinct from Those Responsible for Thymic Selection

Jeremy Benderc, Tom Mitchellb, John Kapplera,c,d, and Philippa Marracka,c,e

a From the Howard Hughes Medical Institute, National Jewish Medical and Research Center, Denver, Colorado 80206
b From the Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206
c Department of Immunology and Medicine, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80206
d Department of Pharmacology, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80206
e Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80206
Howard Hughes Medical Institute, Department of Medicine, K512, National Jewish Center, 1400 Jackson St., Denver, CO 80206.303-398-1396303-398-1307

marrackp{at}njc.org

We investigated the mechanism by which {alpha}/β T cells expand upon transfer to T cell–deficient host mice by injecting carboxyfluorescein diacetate succinimidyl ester–labeled T cells into mice depleted of T cells by sublethal irradiation. We found that CD4+ T cells divided when transferred to irradiated hosts and that the division of more than half of these cells required class II expression. However, division of transferred CD4+ T cells did not occur in irradiated hosts that expressed class II molecules occupied solely by the peptide responsible for thymic selection, indicating that peptides distinct from those involved in thymic selection cause the division of CD4+ T cells in irradiated mice. These data establish that class II–bound peptides control the expansion of CD4+ T cells transferred to T cell–deficient hosts and suggest that the same peptides contribute to the maintenance of T cell numbers in normal mice.

Key Words: T cell homeostasis • peptides • peripheral selection • T cell receptor–major histocompatibility complex interaction • T cell–deficiency

1used in this paper: CFSE, carboxyfluorescein diacetate succinimidyl ester; CyC, cychrome; Ii, invariant chain; KO, knockout; SEB, staphylococcal enterotoxin B

© 1999 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS