Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 87K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gough, L. Articles by Shakib, F. Search for Related Content PubMed PubMed Citation Articles by Gough, L. Articles by Shakib, F. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Social Bookmarking                            What's this?

Brief Definitive Report

^a Division of Molecular and Clinical Immunology, Faculty of Medicine and Health Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom
Div. of Molecular and Clinical Immunology, Faculty of Medicine and Health Sciences, Queen's Medical Centre, Nottingham NG7 2UH, UK.115-9709-125115-9709-123

farouk.shakib{at}nottingham.ac.uk

The house dust mite Dermatophagoides pteronyssinus allergen Der p 1 is the most immunodominant allergen involved in the expression of dust mite–specific immunoglobulin (Ig)E–mediated hypersensitivity. The reason for this potent IgE-eliciting property of Der p 1 remains unknown, but there is mounting in vitro evidence linking the allergenicity of Der p 1 to its cysteine protease activity. Here we demonstrate for the first time that immunization of mice with proteolytically active Der p 1 results in a significant enhancement in total IgE and Der p 1–specific IgE synthesis compared with animals immunized with Der p 1 that was irreversibly blocked with the cysteine protease inhibitor E-64. We conclude that the proteolytic activity of Der p 1 is a major contributor to its allergenicity.

Key Words: cysteine protease • Der p 1 • IgE

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Takai, T., Kato, T., Hatanaka, H., Inui, K., Nakazawa, T., Ichikawa, S., Mitsuishi, K., Ogawa, H., Okumura, K. (2009). Modulation of Allergenicity of Major House Dust Mite Allergens Der f 1 and Der p 1 by Interaction with an Endogenous Ligand. J. Immunol. 183: 7958-7965 [Abstract] [Full Text]  
• Platts-Mills, T. A. E., Erwin, E. A., Heymann, P. W., Woodfolk, J. A. (2009). Pro: The Evidence for a Causal Role of Dust Mites in Asthma. Am. J. Respir. Crit. Care Med. 180: 109-113 [Full Text]  
• Deb, R., Shakib, F., Reid, K., Clark, H. (2007). Major House Dust Mite Allergens Dermatophagoides pteronyssinus 1 and Dermatophagoides farinae 1 Degrade and Inactivate Lung Surfactant Proteins A and D. J. Biol. Chem. 282: 36808-36819 [Abstract] [Full Text]  
• Kikuchi, Y., Takai, T., Kuhara, T., Ota, M., Kato, T., Hatanaka, H., Ichikawa, S., Tokura, T., Akiba, H., Mitsuishi, K., Ikeda, S., Okumura, K., Ogawa, H. (2006). Crucial Commitment of Proteolytic Activity of a Purified Recombinant Major House Dust Mite Allergen Der p1 to Sensitization toward IgE and IgG Responses. J. Immunol. 177: 1609-1617 [Abstract] [Full Text]  
• van Oosterhout, A. J. M., Bloksma, N. (2005). Regulatory T-lymphocytes in asthma. Eur Respir J 26: 918-932 [Abstract] [Full Text]  
• DOUGALL, A., HOLT, D. C., FISCHER, K., CURRIE, B. J., KEMP, D. J., WALTON, S. F. (2005). IDENTIFICATION AND CHARACTERIZATION OF SARCOPTES SCABIEI AND DERMATOPHAGOIDES PTERONYSSINUS GLUTATHIONE S-TRANSFERASES: IMPLICATION AS A POTENTIAL MAJOR ALLERGEN IN CRUSTED SCABIES. Am J Trop Med Hyg 73: 977-984 [Abstract] [Full Text]  
• Kilmon, M. A., Shelburne, A. E., Chan-Li, Y., Holmes, K. L., Conrad, D. H. (2004). CD23 Trimers Are Preassociated on the Cell Surface Even in the Absence of Its Ligand, IgE. J. Immunol. 172: 1065-1073 [Abstract] [Full Text]  
• Pollock, K. G. J., McNeil, K. S., Mottram, J. C., Lyons, R. E., Brewer, J. M., Scott, P., Coombs, G. H., Alexander, J. (2003). The Leishmania mexicana Cysteine Protease, CPB2.8, Induces Potent Th2 Responses. J. Immunol. 170: 1746-1753 [Abstract] [Full Text]  
• Kheradmand, F., Kiss, A., Xu, J., Lee, S.-H., Kolattukudy, P. E., Corry, D. B. (2002). A Protease-Activated Pathway Underlying Th Cell Type 2 Activation and Allergic Lung Disease. J. Immunol. 169: 5904-5911 [Abstract] [Full Text]  
• POMES, A., CHAPMAN, M. D., VAILES, L. D., BLUNDELL, T. L., DHANARAJ, V. (2002). Cockroach Allergen Bla g 2 . Structure, Function, and Implications for Allergic Sensitization. Am. J. Respir. Crit. Care Med. 165: 391-397 [Abstract] [Full Text]  
• Loukas, A., Prociv, P. (2001). Immune Responses in Hookworm Infections. Clin. Microbiol. Rev. 14: 689-703 [Abstract] [Full Text]  
• Furmonaviciene, R, Shakib, F (2001). The molecular basis of allergenicity: comparative analysis of the three dimensional structures of diverse allergens reveals a common structural motif. Mol. Pathol. 54: 155-159 [Abstract] [Full Text]  
• Huecas, S., Villalba, M., Rodriguez, R. (2001). Ole e 9, a Major Olive Pollen Allergen Is a 1,3-beta -Glucanase. ISOLATION, CHARACTERIZATION, AMINO ACID SEQUENCE, AND TISSUE SPECIFICITY. J. Biol. Chem. 276: 27959-27966 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS