The Journal of Experimental Medicine
IN Cell Analyzer 2000
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© The Rockefeller University Press, 0022-1007/1999/12/1617/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 11, December 6, 1999 1617-1626

Original Article

Commitment of Common T/Natural Killer (Nk) Progenitors to Unipotent T and Nk Progenitors in the Murine Fetal Thymus Revealed by a Single Progenitor Assay

Tomokatsu Ikawaa, Hiroshi Kawamotoa, Shinji Fujimotoa, and Yoshimoto Katsuraa

a Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.81-75-751-464881-75-751-3842

katsura{at}frontier.kyoto-u.ac.jp

We have established a new clonal assay system that can evenly support the development of T and natural killer (NK) cells. With this system, we show that all T cell progenitors in the earliest CD44+CD25–Fc{gamma}RII/III– fetal thymus (FT) cell population retain NK potential, and that the NK lineage–committed progenitors (p-NK) also exist in this population. T cell lineage–committed progenitors (p-T), which are unable to generate NK cells, first appear at the CD44+CD25– Fc{gamma}RII/III+ stage in day 12 FT. The proportion of p-T markedly increases during the transition from the CD44+CD25– stage to the CD44+CD25+ stage in day 14 FT. On the other hand, p-NK preferentially increase in number at the CD44+CD25– stage between days 12 and 14 of gestation. The production of p-NK continues up to the CD44+CD25+ stage, but ceases before the rearrangement of T cell receptor β chain genes. It was further shown that the CD44+CD25– CD122+ population of day 14 FT exclusively contains p-NK. These results indicate that the earliest T cell progenitor migrating into the FT is T/NK bipotent, and strongly suggest that the bipotent progenitor continuously produces p-NK and p-T until the CD44+CD25+ stage.

Key Words: T cell • natural killer cell • hematopoietic stem cell • thymus • clonal assay

Abbreviations used in this paper: APC, allophycocyanin; B6, C57BL/6; dGuo, deoxyguanosine; dpc, day(s) postcoitum; FcR, Fc{gamma}RII/III; FL, fetal liver; FT, fetal thymus; HOS, high oxygen submersion; Lin, lineage marker; MLP, multilineage progenitor; p-NK, NK lineage–committed progenitor; p-T, T cell lineage–committed progenitor; p-T/NK, bipotent progenitor generating T and NK cells; SCF, stem cell factor.

© 1999 The Rockefeller University Press


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