Commitment of Common T/Natural Killer (NK) Progenitors to Unipotent T and NK Progenitors in the Murine Fetal Thymus Revealed by a Single Progenitor Assay

doi:10.1084/jem.190.11.1617

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© The Rockefeller University Press, 0022-1007/1999/12/1617/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 11, December 6, 1999 1617-1626

Original Article

Commitment of Common T/Natural Killer (NK) Progenitors to Unipotent T and NK Progenitors in the Murine Fetal Thymus Revealed by a Single Progenitor Assay

Tomokatsu Ikawa^a, Hiroshi Kawamoto^a, Shinji Fujimoto^a, and Yoshimoto Katsura^a
^a Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan

Correspondence to: Yoshimoto Katsura, Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan. Tel:81-75-751-3842 Fax:81-75-751-4648 E-mail:katsura{at}frontier.kyoto-u.ac.jp.

We have established a new clonal assay system that can evenlysupport the development of T and natural killer (NK) cells.With this system, we show that all T cell progenitors in theearliest CD44⁺CD25^-Fc ${gamma}$ RII/III^- fetal thymus (FT) cell populationretain NK potential, and that the NK lineage–committedprogenitors (p-NK) also exist in this population. T cell lineage–committedprogenitors (p-T), which are unable to generate NK cells, firstappear at the CD44⁺CD25^- Fc ${gamma}$ RII/III⁺ stage in day 12 FT. Theproportion of p-T markedly increases during the transition fromthe CD44⁺CD25^- stage to the CD44⁺CD25⁺ stage in day 14 FT. Onthe other hand, p-NK preferentially increase in number at theCD44⁺CD25^- stage between days 12 and 14 of gestation. The productionof p-NK continues up to the CD44⁺CD25⁺ stage, but ceases beforethe rearrangement of T cell receptor ß chain genes. Itwas further shown that the CD44⁺CD25^- CD122⁺ population of day14 FT exclusively contains p-NK. These results indicate thatthe earliest T cell progenitor migrating into the FT is T/NKbipotent, and strongly suggest that the bipotent progenitorcontinuously produces p-NK and p-T until the CD44⁺CD25⁺ stage.

Key Words: T cell, natural killer cell, hematopoietic stem cell, thymus,clonal assay

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