The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/11/1451/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 10, November 15, 1999 1451-1464

Original Article

Differential Regulation of Constitutive Major Histocompatibility Complex Class I Expression in T and B Lymphocytes

Chien-Kuo Leea, Ramon Gimenoa, and David E. Levya

a Department of Pathology and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016
Dept. of Pathology, New York University School of Medicine, 550 First Ave., New York, NY 10016.212-263-8211212-263-8192

levyd01{at}med.nyu.edu

Major histocompatibility complex (MHC) class I antigens are constitutively expressed yet highly induced by interferon (IFN) during inflammation. We found that not only IFN-induced but also normal basal expression of MHC I required IFN receptors and signal transducer and activator of transcription (STAT)1, providing genetic evidence for continuous IFN signaling. Surprisingly, an IFN-independent requirement for STAT1 was also found, specifically in T lymphocytes, where MHC class I expression was not fully accounted for by IFN signaling. This IFN-independent pathway maintained tyrosine phosphorylation of STAT1 in T but not B lymphocytes even in the absence of IFN receptors. Interestingly, interleukin (IL)-7 selectively activated STAT1 and induced MHC class I in mature T but not B cells. These loss of function studies demonstrate an essential role of endogenous IFN and activated STAT1 for constitutive MHC class I expression in normal mice and define IL-7–dependent but IFN-independent regulation of STAT1 restricted to T lymphocytes.

Key Words: interferon • STAT1 • interleukin-7 • gene regulation • tyrosine phosphorylation

1used in this paper: BMT, bone marrow transplantation; DN, double-negative; DP, double-positive; EMSA, electrophoretic mobility gel shift; GFP, green fluorescent protein; ICS, IFN consensus sequence; IRF, IFN regulatory factor; NF, nuclear factor; PML, promyelocytic leukemia; SP, single-positive; STAT, signal transducer and activator of transcription; TAP, transporter associated with antigen processing

© 1999 The Rockefeller University Press


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