The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/11/1375/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 10, November 15, 1999 1375-1382

Original Article

A Proinflammatory Cytokine Inhibits P53 Tumor Suppressor Activity

James D. Hudsona, Mahmood A. Shoaibia, Roberta Maestrob, Amancio Carneroa, Gregory J. Hannonc, and David H. Beacha

a Unit of Cancer Biology, Institute of Child Health, London WC1N 1EH, United Kingdom
b Experimental Oncology 1, Centro di Riferimento Oncologico, 33081 Aviano, Italy
c Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
Institute of Child Health, University College London, Cruciform Building, Gower Street, London WC1E 6BT, UK.171-813-0358171-813-8495

D.Beach{at}ich.ucl.ac.uk

p53 has a key role in the negative regulation of cell proliferation, in the maintenance of genomic stability, and in the suppression of transformation and tumorigenesis. To identify novel regulators of p53, we undertook two functional screens to isolate genes which bypassed either p53-mediated growth arrest or apoptosis. In both screens, we isolated cDNAs encoding macrophage migration inhibitory factor (MIF), a cytokine that was shown previously to exert both local and systemic proinflammatory activities. Treatment with MIF overcame p53 activity in three different biological assays, and suppressed its activity as a transcriptional activator. The observation that a proinflammatory cytokine, MIF, is capable of functionally inactivating a tumor suppressor, p53, may provide a link between inflammation and tumorigenesis.

Key Words: macrophage migration inhibitory factor • p53 • inflammation and cancer • growth arrest • apoptosis

1used in this paper: FBS, fetal bovine serum; GFP, green fluorescent protein; GSNO, S-nitrosoglutathione; MBP, maltose binding protein; MEF, mouse embryonic fibroblast; MIF, macrophage migration inhibitory factor; NO, nitric oxide; SNP, sodium nitroprusside

© 1999 The Rockefeller University Press


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