Stem Cell Emergence and Hemopoietic Activity Are Incompatible in Mouse Intraembryonic Sites

doi:10.1084/jem.190.1.43

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© The Rockefeller University Press, 0022-1007/1999/7/43/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 1, July 1, 1999 43-52

Original Article

Stem Cell Emergence and Hemopoietic Activity Are Incompatible in Mouse Intraembryonic Sites

Isabelle Godin^a, Juan Antonio Garcia-Porrero^b, Françoise Dieterlen-Lièvre^a, and Ana Cumano^c

^a From the Institut d'Embryologie Cellulaire et Moléculaire du Centre National de la Recherche Scientifique (CNRS) et du Collège de France, 94736 Nogent sur Marne Cedex, France
^b Departamiento de Anatomia y Biologia Cellular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain
^c Unité de Biologie Moléculaire du Gène et Unité du Développement des Lymphocytes, Institut Pasteur, 75024 Paris Cedex 15, France

In the mouse embryo, the generation of candidate progenitorsfor long-lasting hemopoiesis has been reported in the paraaorticsplanchnopleura (P-Sp)/aorta-gonad-mesonephros (AGM) region.Here, we address the following question: can the P-Sp/AGM environmentsupport hemopoietic differentiation as well as generate stemcells, and, conversely, are other sites where hemopoietic differentiationoccurs capable of generating stem cells? Although P-Sp/AGM generatesde novo hemopoietic stem cells between 9.5 and 12.5 days postcoitus (dpc), we show here that it does not support hemopoieticdifferentiation. Among mesoderm-derived sites, spleen and omentumwere shown to be colonized by exogenous cells in the same fashionas the fetal liver. Cells colonizing the spleen were multipotentand pursued their evolution to committed progenitors in thisorgan. In contrast, the omentum, which was colonized by lymphoid-committedprogenitors that did not expand, cannot be considered as a hemopoieticorgan. From these data, stem cell generation appears incompatiblewith hemopoietic activity. At the peak of hemopoietic progenitorproduction in the P-Sp/AGM, between 10.5 and 11.5 dpc, multipotentcells were found at the exceptional frequency of 1 out of 12total cells and 1 out of 4 AA4.1⁺ cells. Thus, progenitors withinthis region constitute a pool of undifferentiated hemopoieticcells readily accessible for characterization.

Key Words: aorta-gonad-mesonephros • spleen • omentum • hemopoiesis • reconstitution

1used in this paper: AGM, aorta-gonad-mesonephros; BFU-E, burst-formingunit–erythroid; CFC, colony-forming cell; CFU-S, colony-formingunit–splenic; dpc, day(s) post coitus; Epo, erythropoietin;FTOC, fetal thymic organ culture; HSC, hemopoietic stem cell;KL, c-Kit ligand; LTR, long-term reconstitution; PeC, peritonealcavity; P-Sp, paraaortic splanchnopleura; YS, yolk sac

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