The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/1999/5/1507/ $5.00
The Journal of Experimental Medicine, Volume 189, Number 9, May 3, 1999 1507-1512

Brief Definitive Reports

Humoral and Cell-mediated Autoimmune Reactions to Human Acidic Ribosomal P2 Protein in Individuals Sensitized to Aspergillus fumigatus P2 Protein

Christina Mayer*, Ulrich Appenzeller*, Heike Seelbach{ddagger}, Gernot Achatz§, Hannes Oberkofler§, Michael Breitenbach§, Kurt Blaser*, and Reto Crameri*

From the * Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland; {ddagger} Hochgebirgsklinik, CH-7265 Davos-Wolfgang, Switzerland; and the § Institut für Genetik und Allgemeine Biologie, Universität Salzburg, A-5020 Salzburg, Austria

A panel of cDNAs encoding allergenic proteins was isolated from an Aspergillus fumigatus cDNA library displayed on the surface of filamentous phage. Solid phase–immobilized serum immunoglobulin E (IgE) from A. fumigatus–allergic individuals was used to enrich phage displaying IgE-binding molecules. One of the cDNAs encoded a 11.1-kD protein that was identified as acidic ribosomal phosphoprotein type 2 (P2 protein). The allergen, formally termed rAsp f 8, shares >62% sequence identity and >84% sequence homology to corresponding eukaryotic P2 proteins, including human P2 protein. The sequences encoding human and fungal P2 protein were subcloned, expressed in Escherichia coli as His6-tagged fusion proteins, and purified by Ni2+–chelate affinity chromatography. Both recombinant P2 proteins were recognized by IgE antibodies from allergic individuals sensitized to the A. fumigatus P2 protein and elicited strong type 1–specific skin reactions in these individuals. Moreover, human and fungal P2 proteins induced proliferative responses in peripheral blood mononuclear cells of A. fumigatus– allergic subjects sensitized to the fungal P2 protein. These data provide strong evidence for in vitro and in vivo humoral and cell-mediated autoreactivity to human P2 protein in patients suffering from chronic A. fumigatus allergy.

Key Words: phage display • cDNA libraries • IgE • allergens • autoimmunity

Address correspondence to Reto Crameri, Swiss Institute of Allergy and Asthma Research (SIAF), Obere Strasse 22, CH-7270 Davos Platz, Switzerland. Phone: 41-81-410-08-48; Fax: 41-81-410-08-40; E-mail: crameri{at}siaf.unizh.ch


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