J. Exp. Med., Volume 189, Number 8, April 19, 1999 1169-1180

Hapten-induced Colitis Is Associated with Colonic Patch Hypertrophy and T Helper Cell 2-Type Responses

By Taeko Dohi,^* Kohtaro Fujihashi,^* Paul D. Rennert,^§ Koichi Iwatani,^¶ Hiroshi Kiyono,^*¶ and Jerry R. McGhee^*

From the ^* Immunobiology Vaccine Center, Department of Microbiology and  Department of Oral Biology,  The University of Alabama at Birmingham, Birmingham, Alabama 35294; ^§ Biogen Incorporated, Cambridge, Massachusetts 02142; and the ^¶ Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan

To investigate the potential involvement of T helper (Th)2-type responses in murine models of intestinal inflammation, we used trinitrobenzene sulfonic acid (TNBS)-hapten to induce inflammatory bowel disease in situations where Th1-type responses with interferon (IFN)- synthesis are either diminished or do not occur. Intracolonic administration of TNBS to either normal (IFN-^+/+) or Th1-deficient IFN- knockout (IFN-^/) BALB/c mice resulted in significant colitis. In IFN-^/ mice, crypt inflammation was more severe than in IFN-^+/+ mice and was accompanied by hypertrophy of colonic patches with a lymphoepithelium containing M cells and distinct B and T cell zones resembling Peyer's patches. Hapten-specific, colonic patch T cells from both mouse groups exhibited a Th2 phenotype with interleukin (IL)-4 and IL-5 production. TNBS colitis in normal mice treated with anti-IL-4 antibodies or in IL-4^/ mice was less severe than in either IFN-^+/+ or IFN-^/ mice. Our findings now show that the Th2-type responses in TNBS colitis are associated with colonic patch enlargement and inflammation of the mucosal layer and may represent a model for ulcerative colitis.

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