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Department of Pediatrics; and the
Department of Pathology, Stanford University School of Medicine, Stanford, California 94305
Sorted CD4+ and CD8+ T cells from the peripheral blood or bone marrow of donor C57BL/6 (H-2b) mice were tested for their capacity to induce graft-versus-host disease (GVHD) by injecting the cells, along with stringently T cell–depleted donor marrow cells, into lethally irradiated BALB/c (H-2d) host mice. The peripheral blood T cells were at least 30 times more potent than the marrow T cells in inducing lethal GVHD. As NK1.1+ T cells represented <1% of all T cells in the blood and
30% of T cells in the marrow, the capacity of sorted marrow NK1.1– CD4+ and CD8+ T cells to induce GVHD was tested. The latter cells had markedly increased potency, and adding back marrow NK1.1+ T cells suppressed GVHD. The marrow NK1.1+ T cells secreted high levels of both interferon
(IFN-
) and interleukin 4 (IL-4), and the NK1.1– T cells secreted high levels of IFN-
with little IL-4. Marrow NK1.1+ T cells obtained from IL-4–/– rather than wild-type C57BL/6 donors not only failed to prevent GVHD but actually increased its severity. Together, these results demonstrate that GVHD is reciprocally regulated by the NK1.1– and NK1.1+ T cell subsets via their differential production of cytokines.
Key Words: bone marrow transplantation immune regulation regulatory T cells cytokines interleukin 4
Abbreviations used: TCD, T cell–depleted.
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